APHIS Freedom of Information Act (FOIA) Appeal Mouse Bio-Assays
2007-00030-A Sheep Imported From Belgium and the Presence of TSE Prion Disease
Kevin Shea to Singeltary 2015
Greetings BSE-L Members et al,
you can’t believe what I got in the US Postal mail today. the wife would
not pick it up yesterday, because there was a $6.00 charge for a certified
letter from USDA Kevin Shea for about 5 pages. I went to the PO today, told the
girls in the back that if it’s an affidavit, a warrant, summons, I don’t want
it, send it back. but it was certified. scared me. but the curiosity got to me,
so i coughed up 6 bucks, and took a chance. low and behold, after my last appeal
to this decade plus old quest was turned down, even though I already had the
answer from another source, APHIS et al finally stumbled across those old mouse
bio-assays. they had them all along.
what the industry sent me first was better, because it had some of the good
stuff i.e. redacted.
this all started way back around the year 2,000, when in my opinion, the
USDA et al let these sheep in the USA from Belgium, when they should not have
because of atypical BSE in Belgium. I started asking for the these mouse
bio-assays back in or around 2003 or before, then I had to get official with
FOIA, because no one would answer my questions.
well, it’s February 20, 2015, over a decade later, and I don’t know how
many denials, here’s what was in the mail yesterday, February 19, 2015 ;
United States Department of Agriculture
Animal and Plant Health Inspection Service Marketing and Regulatory
Programs Animal and Plant Health Inspection Service Legislative and Public
Affairs Freedom of Information 4700 River Road Unit 50 Riverdale, MD. 20737-1232
FEB 10 2015
Terry S. Singletary Sr. P.O. Box 42 Bacliff, Texas 77518
Re: FOIA Appeal # 2007-00030-A
Dear Mr. Singletary:
This letter is in response to the Freedom of Information Act (FOIA) appeal
that you submitted regarding FOIA request 07-566. Your appeal challenged the
APHIS FOIA Office's search for the "Mouse Bio-Assays" on the sheep imported from
Belgium. We apologize for the delayed response.
The APHIS FOIA Office received your appeal, on July 7, 2007 and assigned it
FOIA case number 2007-00030-A.
In response to your appeal, the APHIS FOIA Office performed a second search
of records responsive to your initial request. The Agency has since found four
(4) pages of responsive records for the "Mouse Bio-Assays" dated October
22,2009. Although these records postdate both your initial request and
subsequent appeal by approximately two years; we enclose them in the interest of
responsiveness to your request.
We now consider this appeal closed and will take no further action. If you
are dissatisfied with this decision, you have the right to judicial review in an
appropriate United States District Court in accordance with 5 U.S.C. 552,
(2)(4)(B).
Prior to seeking judicial review, you may contact the Office of Government
Information Services (OGlS). OGIS was created within the National Archives and
Records Administration when the Open Government Act of 2007 amended the FOIA.
OGIS provides mediation of FOIA disputes between appellants and federal
agencies. Participation in mediation does not affect your right to judicial
review. Contact information for OGIS can be found at
http:/www.archives.gov/ogis.
Sincerely,
Kevin Shea Administrator Enclosure
snip...end
the next 4 pages is exactly what I received from an industry source way
back on Saturday, February 27, 2010. see ;
Saturday, February 27, 2010
see history below ;
From: Terry S. Singeltary Sr. Sent: Thursday, February 19, 2015 10:04 PM
To: Terry S. Singeltary Sr. Subject: mad sheep mad river valley...NOT...never
was
Veterinary Laboratories Agency – Weybridge
New Haw, Addlestone, Surrey KT15 3NB United Kingdom
Telephone +44 (0)1932 341111 Facsimile +44 (0)1932 347046 '
Veterinary Laboratories Agency
Your ref: MPL-6197-7-37
Our ref: FT1294
This is the FINAL report for contract MPL-6197-7-37 The testing of the
Belgian (Vermont) sheep.
Background
Brain homogenate (10% in normal saline) from each case was inoculated
intracerebralty into panels of 20 Rlll and 20 Tg338 mice.
FT1294/0001 (Sample 4677) was inoculated into mice on the 14/12/06
FT1294/0011 (Sample 4703) was inoculated into mice on the 20/12/06
Method
The brain from each mouse was examined histologically for any evidence of
TSE-related vacuolation, and immunolabelled using anti-PrP antibody Rb486 as
described elsewhere1, All slide interpretation was undertaken blind with regard
to the clinical status of the mouse, or the source of the inoculum.
Final bioassay results
FT1294/0001 (Sample 4677)
Tg338 mice - All 20 mice are negative by histopathology, and
immunohistochemistry
Rlll mice - All 20 mice are negative negative by histopathology, and
immunohistochemistry
FT1294/0011 (Sample 4703)
Tg338 mice - All 20 mice are negative by histopathology, and
immunohistochemistry
Rlll mice - All 20 mice are negative by histopathology, and
immunohrstochernistry
The survival times for these mice can be seen in the figures below.
Additional data sets from positive and negative inocula (J Spiropoulos, pers.
comm.) have been included for comparison._
1 Beck KE, Chaplin M, Stack M: Sallis RE, Simonini S, Lockey R, Spiropoulos
J. Lesion Profiling at Primary Isolation in Rlll Mice Is Insufficient in
Distinguishing BSE from Classical Scrapie. Brain Pathol. 2009 epub ahead of
print
FINAL report for contract MPL-6197-7-37
VLA is an Executive Agency of the Department for Environment, Food and
Rural Affairs (Defra)
snip... (2 pages of charts and graphs of survival and comparison of tg338
data NOT included here...TSS)
CONCLUSION
These mice have survived for long enough to have demonstrated the presence
of classical scrapie, atypical scrapie, or ovine BSE if any of these strains was
present in the inoculum.
Both samples are negative by bioassay.
Dr. Marion M Simmons
22nd October 2009
February 27, 2010, INVESTIGATION OF MAD SHEEP OF MAD RIVER VALLEY COMPLETE.
THEY WERE NOT INFECTED WITH ANY TSE. ...TSS
Greetings again BSE list members,
The investigation of the Mad Sheep of Mad River Valley may be complete now,
but, I still have questions.
PLEASE SEE MY FINAL FOIA HERE ;
Monday, September 1, 2008
RE-FOIA OF DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL
T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [No. 00-072-1]
September 1, 2008
Greetings again BSE-L members,
I had a pleasant surprise this past Saturday. I got an unexpected package
from O.I.G. on my old F.O.I.A. request, of the final test results of the
infamous mad sheep of mad river valley. IF you all remember, back on Thu, 24 Apr
2008 15:00:20 -0500 I wrote ;
Greetings,
With great disgust, I must report, that after years and years of wrangling
over the infamous mad sheep of mad river valley, I have failed in getting an
official answer via FOIA on the outcome of the TSE testing of those imported
Belgium sheep. The USA Government refuses to tell the public, exactly what the
testing outcome was, and in doing so, shows just how corrupt this administration
has been. and the excuse given in their answer to my final appeal, which they
have now officially denied, was bizarre to say the least ;
"I am denying your FOIA appeal. This is the final agency decision. You may
seek judicial review of this decision in the United States district court for
the judicial district in which you reside or have your principal place of
business or in the District of Columbia, pursuant to 5 U.S.C. &
552(a)(4)(B)."
FOIA OF DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL
T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No.
00-072-1] ...snip...end...TSS
NOW, out of the wild blue, AFTER them telling me they denied my FOIA appeal
for the final time, any further action would have to be judicial review in the
United States district court, I get 25+ pages, a lot of redacted names, etc, but
this is the first time they sent me anything about this in the 6 years of
waiting for my FOIA request. IT will take me a long time to get this online due
to the fact you cannot hardly read it, very poor quality and eligibility of
text. BUT, the just of it is, somebody (REDACTED) screwed those tests up. I will
work to get all the data online next week or so, but it is odd how much they
were concerned for human and animal health from an atypical scrapie of foreign
origin back then, but yet when we document it here in the USA, you don't hear a
word about it. it's a completely different story.
IN SHORT ;
August 15, 2000
OIG case # NY-3399-56 REDACTED, VT
''Enclosed is OIG's notification that they have scheduled an investigation
of the following individual. REDACTED is alleged to have provided possibly
inaccurate test results involving diseased sheep. However, because the results
were determined to be inconclusive, no actual violation was actually
committed.''
snip...
IN SHORT ;
August 15, 2000
OIG case # NY-3399-56 REDACTED, VT
''Enclosed is OIG's notification that they have scheduled an investigation
of the following individual. REDACTED is alleged to have provided possibly
inaccurate test results involving diseased sheep. However, because the results
were determined to be inconclusive, no actual violation was actually
committed.''
snip...
[only bush et al could have interpreted it that way. don't all criminals
wish this is the way the system worked. ...tss]
JULY, 28, 2000
Case Opening Memorandum
snip...
An investigation regarding the subject identified below will be conduced
and a report submitted at the conclusion of the investigation. If you have or
should later receive additional information concerning this matter, please
forward it to this office.
If you believe that administrative action should be taken before all
criminal and other legal matters are completed, please coordinate that action
with this office in order not to jeopardize the ongoing investigation.
The fact that this subject is under investigation should not be discussed
with anyone who does not have a need to know and all inquiries on the
investigation should be referred to the office of Inspector General.
snip...end
FOR OFFICIAL USE ONLY FEBRUARY 7, 2002
SUBJECT OIG CASE NY-3399-56 REDACTED VT HEALTH/SANITATION VIOLATION
TO: William Buisch, Regional Director Eastern Region, VS Raleigh, NC
Enclosed is the official investigation report on REDACTED. If you will
recall, REDACTED is alleged to have provided possible inaccurate test results
involving diseased sheep.
OIG is closing their file upon issuance of the Report of Investigation
(copy enclosed). We are, therefore, also closing our case file.
REDACTED
Resource Management Systems and Evaluation Staff
Enclosure
cc:
REDACTED IES, Riverdale, MD (w/cy of incoming)
APHIS:RMSES: REDACTED 2/7/02 "NY-3399-56-REDACTED Closure''
END...TSS
NOW, the question is, who screwed those test up, and was it done on
purpose, just to cover someone's ass for letting those sheep in here in the
first place ???
WHICH tests were compromised, one of them, all of them, and, can we trust
the outcome of any of these test under the circumstances here ???
i.e.
"It is significant that four of the sheep which first tested positive on
REDACTED Western blot tests, thereby providing the type of confirmation the
plaintiffs argue is lacking on the current record."
UNDER what circumstances were these test compromised ???
MY basic, simple question, was not answered in layman term, i.e. exactly
what strain of TSE did those sheep have ???
IS this the best we can do ???
>>>"REDACTED is alleged to have provided possibly inaccurate test
results involving diseased sheep. However, because the results were determined
to be inconclusive, no actual violation was actually committed.''<<<
PLEASE SEE FULL TEXT HERE ;
Saturday, February 27, 2010
*** FINAL REPORT OF THE TESTING OF THE BELGIAN (VERMONT) SHEEP February 27,
2010 IN SHORT ; August 15, 2000 OIG case # NY-3399-56 REDACTED, VT ''Enclosed is
OIG's notification that they have scheduled an investigation of the following
individual. REDACTED is alleged to have provided possibly inaccurate test
results involving diseased sheep. However, because the results were determined
to be inconclusive, no actual violation was actually committed.''
FINAL REPORT OF THE TESTING OF THE BELGIAN (VERMONT) SHEEP February 27,
2010
(10 YEARS LATER, FOIA, none of the sheep had any TSE at all...tss)
Thursday, April 24, 2008
RE-FOIA OF DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL
T.S.E. OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No. 00-072-1]
Monday, September 1, 2008 RE-FOIA OF DECLARATION OF EXTRAORDINARY EMERGENCY
BECAUSE OF AN ATYPICAL T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED
STATES [No. 00-072-1]
FOIA MAD SHEEP MAD RIVER VALLEY
Tuesday, November 13, 2007
DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. (PRION
DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No. 00-072-1]
To: Garfield.O.Daley@aphis.usda.gov
CC: phyllis.Fong@usda.gov; bse-L@aegee.org;
Re: FOIA APPEAL 07-566 DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN
ATYPICAL T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [Docket
No. 00-072-1]
November 13, 2007
Greetings Garfield O. Daley, Acting FOIA Director, and USDA et al,
SNIP
for those interested, please see full text answer below received from USDA
et al below on latest appeal ;
Tuesday, November 13, 2007
DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. (PRION
DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No. 00-072-1]
To: Garfield.O.Daley@aphis.usda.gov
CC: phyllis.Fong@usda.gov; bse-L@aegee.org;
Re: FOIA APPEAL 07-566 DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN
ATYPICAL T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [Docket
No. 00-072-1]
EXACTLY WHAT are these people capable of doing ???
JUST HOW FAR will they go ???
Mad Sheep The True Story Behind the USDA‚ War on a Family Farm Linda
Faillace
The page-turning account of a government cover-up, corporate greed, and a
courageous family‚ fight to save their farm.
----- Original Message -----
From: Terry S. Singeltary Sr.
Sent: Sunday, February 25, 2007 12:35 PM
Subject: FOIA REQUEST FOR ATYPICAL TSE INFORMATION ON VERMONT SHEEP
Greetings USDA,
I respectfully request the final results of the mouse bio-assays test that
were to have supposedly began 2+ years late, 5 years ago, on the imported sheep
from Belgium ?
WHAT happened to the test results and MOUSE BIO-ASSAYS of those imported
sheep from Belgium that were confiscated and slaughtered from the Faillace's,
what sort of TSE did these animals have ?
WERE they atypical scrapie, BSE, and or typical scrapie ?
HOW much longer will you refuse to give us this information ? and for what
reason ?
WHY is it that the Farm of the Mad Sheep of Mad River Valley were
quarantined for 5 years, but none of these farms from Texas and Alabama with
Atypical TSE in the Bovine, they have not been quarantined for 5 years, why not,
with the real risk of BSE to sheep, whom is to say this was not BSE ?
snip.
full text ;
UNITED STATES DEPARTMENT OF AGRICULTURE OFFICE OF INSPECTOR GENERAL
WASHINGTON D.C. 20250
DEC 28, 2007
Mr. Terry S. Singeltary, Sr. P.O. Box 42 Bacliff, Texas 77518
Subject: FOIA Appeal-Log No. 08-00034 (No. 07-00060)
Dear Mr. Singeltary:
This is in response to your December 3, 2007, Freedom of Information Act
(FOIA), 5 U.S.C. & 552, appeal of the November 20, 2007, decision of Ms.
Deirdre MacNeil, FOIA/Privacy Act (PA) Attorney, Office of Inspector General
(OIG), Department of Agriculture (USDA). As explained below, your FOIA appeal is
denied.
As background, on March 1, 2007, you requested the "final results of the
TSE Mouse-bioassays of those Atypical TSE in the Vermont Sheep." FOIA requires
the release of agency records except where one or more of the nine enumerated
exceptions apply. On November 20, 2007, Ms. MacNeil responded to your request by
sending you seven pages from Hotline files PS-3340-0024, which was responsive to
your request. Ms. MacNeil withheld identifying information pursuant to
Exceptions 6 and 7(C) of the FOIA. See 5. U.S.C.& 552(b)(6) and (7)(C). On
December 3, 2007, you appealed Ms. MacNeils decision.
12-3-07
To The Honorable Inspector General USDA,
I respectfully "APPEAL" the decision to withhold information I requested
under the F.O.I.A. About the final results of the T.S.E. Mouse-bioassays of the
Atypical T.S.E. in the Vermont Sheep imported from Belgium and later confiscated
and slaughtered under a "Extra Ordinary Declaration of Emergency due to Atypical
T.S.E. in U.S.A. sheep.
Log Number 07-00060 FOIA 07-566
Terry S. Singeltary Sr. P.O. Box 42 Bacliff, Texas USA 77518
Exemption 6 permits the Government to withhold information about
individuals in "personnel and medical files and similar files the disclosure of
which would constitute a clearly unwarranted invasion of personal privacy." 5
U.S.C. & 552 (b)(6). To warrant protection under Exemption 6, information
must first meet a threshold requirement by falling within the category of
personnel and medical files and similar files. Id. Information fits into a
"similar file" if it contains information regarding a particular individual. See
United States Dept of State V. Washington Post Co., 456, 601-02 (1982). The
threshold is met in this case, as the memorandum contains information regarding
particular named individuals.
Exemption 7(C) protects from disclosure law enforcement information, the
disclosure of which "could reasonably be expected to constitute and unwarranted
invasion of personal privacy." 5 U.S.C. & 552(b)(7)(C. Under Exemption 7(C),
it has been held that a protectible privacy interest exists in the identities of
investigative agents. See Senate of
Mr. Terry S. Singeltary, Sr. Page 2
Puerto Rico v. United States Dep't of Justice, 823 F.2d 574, 588-89 (D.C.
Cir. 1987); Nishnic v. United States Dep't of Justice, 671 F. Supp. 776, 789
(D.D.C. 1987). Such a privacy interest exists in this case, as the withheld
information contains the identities, including names and identifying
information, of investigative agents in the memorandum.
Once it is determined that a privacy interest exists, Exemptions 6 and
7(C), of FOIA require a balancing of interests between the public interest
served by disclosure and an individual's right to privacy. See, e.g., Senate of
Puerto Rico, 823 F.2d at 587; Dep't of the Air Force v. Rose, 425 U.S. 352, 372
(1976). Determination of whether disclosure is warranted turns not upon the
particular purpose for which the document is requested, but upon the nature of
the requested document and its relationship to the central purpose of FOIA,
which is to "open agency action to the light of public scrutiny." United States
Dep't of Justice v. Reporters Comm. for Freedom of the Press, 489 U.S. 749,
772-73 (1989) (quoting Rose, 425 U.S. at 372). I have determined that the
release of the withheld information, of investigative agents in the memorandum,
would not serve the public interest. Therefore, I am denying your appeal with
respect to the withholdings pursuant to Exemptions 6 and 7(C).
In addition to appealing the exemptions pursuant to 6 and 7(C), you appear
to take issue with USDA's Animal $ Plant Health Inspection Service's (APHIS)
response to your FOIA requests with APHIS. You may contact APHIS regarding the
status of any such requests by contacting Mr. Garfield Daley, Acting FOIA
Officer, at (301)734-5273, 4700 River Road, Unit 50, Riverdale, MD,
20737-1232
Finally, in your appeal, you seek answers to a series of questions posed to
various USDA officials, including the Inspector General, However, FOIA allows
requesters to access records only. It does not require Federal agencies to
answer questions, render opinions, provide subjective evaluations, or create
explanatory materials, See, e.g., NLRB v. Sears, Roebuck & Co. 421 U.S. 132,
162 (1975); Zemansky v. Epa, 767 f2d 569, 574 (9th Cir. 1985); Flowers v. IRS,
307 F. Supp. 2d 60, 71 (D.D.C. 2004); Citizens Progressive Alliance v. U.S.
Bureau of Indian Affairs, 241 F. Supp. 2d 1342, 1364-65 (D.N.M. 2002); Hudgins
v. IRS, 620 F. Supp. 19, 21 (D.D.C. 1985). As FOIA requires an agency only to
produce responsive non-exempt records to a requester, OIG is not obligated to
answer questions regarding the TSE occurrence as you requested. Therefore, I am
denying your appeal with respect to your questions.
Mr. Terry S. Singeltary, Sr. Page 3
For these reasons, I am denying your FOIA appeal. This is the final agency
decision. You may seek judicial review of this decision in the United States
district court for the judicial district in which you reside or have your
principal place of business or in the District of Columbia, pursuant to 5 U.S.C.
& 552(a)(4)(B).
Sincerely,
David R. Gray
FOR
Phyllis K. Fong
Inspector General
=======END...TSS...4.24.08=======
----- Original Message -----
From: Terry S. Singeltary Sr.
To: Boyd.Rutherford@usda.gov
Sent: Sunday, February 25, 2007 12:35 PM
Subject: FOIA REQUEST FOR ATYPICAL TSE INFORMATION ON VERMONT SHEEP
Greetings USDA,
I respectfully request the final results of the mouse bio-assays test that
were to have supposedly began 2+ years late, 5 years ago, on the imported sheep
from Belgium ?
WHAT happened to the test results and MOUSE BIO-ASSAYS of those imported
sheep from Belgium that were confiscated and slaughtered from the Faillace's,
what sort of TSE did these animals have ?
WERE they atypical scrapie, BSE, and or typical scrapie ?
HOW much longer will you refuse to give us this information ? and for what
reason ?
WHY is it that the Farm of the Mad Sheep of Mad River Valley were
quarantined for 5 years, but none of these farms from Texas and Alabama with
Atypical TSE in the Bovine, they have not been quarantined for 5 years,why not,
with the real risk of BSE to sheep, whom is to say this was not BSE ?
snip...
full text ;
FURTHERMORE, I respectfully request up front, that any fees for this FOIA
be wavered due to the fact this information should be free to the public and is
in the best interest for the public to have these final results, no financial
gain from this FOIA information is to be made either. ...
Thank You,
kind regards,
Terry S. Singeltary Sr.
P.O. Box 42
Bacliff, Texas USA 77518
Imported
Belgium/Netherlands
Sheep Test Results
Background
Factsheet
Veterinary Services April 2002
APHIS
snip...
Additional tests will be conducted to determine exactly what TSE the
animals have BSE or scrapie. These tests involve the use of bioassays that
consist of injecting mice with tissue from the infected animals
Page 15 of 98
8/3/2006
and waiting for them to develop disease. This testing may take at least 2
to 3 years to complete.
DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E.
(PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
(PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [2]
Imported Belgium/Netherlands Sheep Test Results Background Factsheet
Veterinary Services April 2002 APHIS
snip...
Additional tests will be conducted to determine exactly what TSE the
animals have BSE or scrapie. These tests involve the use of bioassays that
consist of injecting mice with tissue from the infected animals
Page 15 of 98
8/3/2006
and waiting for them to develop disease. This testing may take at least 2
to 3 years to complete.
DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. (PRION
DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E (PRION
DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [2]
> > DEPARTMENT OF AGRICULTURE
> >
> > Office of the Secretary
> >
> > [Docket No. 00-072-2]
> >
> > Declaration of Emergency Because of an Atypical
Transmissible
> > Spongiform Encephalopathy (Prion Disease) of Foreign
Origin
> >
> > A transmissible spongiform encephalopathy (TSE) (prion
disease) of
> > foreign origin has been detected in the United States. It is
different
> > from TSE's previously diagnosed in the United States. The TSE
was
> > detected in the progeny of imported sheep. The imported sheep
and
> > their progeny are under quarantine in Vermont.
Transmissible
> > spongiform encephalopathies are degenerative fatal diseases
that can
> > affect livestock. TSE's are caused by similar, as yet
uncharacterized,
> > agents that usually produce spongiform changes in the
brain.
> > Post-mortem analysis has indicated positive results for an
atypical
> > TSE of foreign origin in four sheep in Vermont. Because of
the
> > potentially serious consequences of allowing the disease to
spread to
> > other livestock in the United States, it is necessary to seize
and
> > dispose of those flocks of sheep in Vermont that are affected
with or
> > exposed to the disease, and their germ plasm. The existence of
the
> > atypical TSE of foreign origin represents a threat to U.S.
livestock.
> > It constitutes a real danger to the national economy and a
potential
> > serious burden on interstate and foreign commerce. APHIS
has
> > insufficient funds to carry out the seizure and disposal of
animals
> > and germ plasm necessary to eliminate this disease risk. These
funds
> > would be used to compensate the owners of the animals and germ
plasm
> > for their seizure and disposal in accordance with 21 U.S.C.
134a.
> > Therefore, in accordance with the provisions of the Act of
September
Page 16 of 98
8/3/2006
> > 25, 1981, as amended (7 U.S.C. 147b), I declare that there is
an
> > emergency that threatens the livestock industry of this
country and
> > hereby authorize the transfer and use of such funds as may
be
> > necessary from appropriations or other funds available to
agencies or
> > corporations of the United States Department of Agriculture to
seize
> > and dispose of animals that are affected with or exposed to
this TSE,
> > and their germplasm, in accordance with 21 U.S.C. 134a.
> >
> > Dated: This declaration of emergency shall become effective
July 14,
> > 2000. Dan Glickman, Secretary of Agriculture. [FR Doc.
00-18368 Filed
> > 7-19-00; 8:45 am] BILLING CODE 3410-34-P
>
>
> >
> > I was told that ;
> >
> >
-------- Original Message --------
Subject:
Re: AW: [BSE-L] USDA did not test possible mad cows - Dr.
Detwiler, what about those sheep?
Date: Sun, 13 Jun 2004 11:27:24
-0500
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform
Encephalopathy
To: BSE-L@uni-karlsruhe.de
References:
<13 .2d20eaae.2df84fb9="" aol.com="">
<40c8c7a0 .1080107="" wt.net="">
######## Bovine Spongiform Encephalopathy
#########
Greetings
list members,
Thought I
should let the list know that Dr. Detwiler kindly replied to my
question
about the delayed 'atypical' TSE testing in the Vermont sheep and
tried to
explain what caused the delay. If I interpreted it correctly,
seems it was
the fault of the U.K. ;
--------
Original Message --------
Subject: Sheep
Date: Sat, 12 Jun 2004 14:26:04
EDT
From: LAVET22@aol.com
To: flounder@wt.net
Mr.
Singeltary.
I hope this
finds you well. As you are aware I left the USDA last
year. I can only update
you on the sheep before that time. Contact was
established with the UK on
doing the bioassay studies. They agreed.
However, we were prioritized after
their own needs, hence the delay. I
am aware that there are now additional
labs in Europe running the mouse
bioassay strain typing. You will have to
contact USDA for further word.
Linda
Detwiler
=========
My reply to
Dr. Detwiler;
-------- Original Message --------
Subject: Re:
Sheep
Date: Sat, 12 Jun 2004 13:53:57 -0500
From: "Terry S. Singeltary
Sr."
To: LAVET22@aol.com
References:
<54 .2bd2ac1e.2dfca4bc="" aol.com="">
hello Dr.
Detwiler,
thanks for
your kind reply.
>
However, we were prioritized after their own needs, hence the
delay.
not sure i
understand that?
> You
will have to contact USDA for further word.
already
done that, and there answer was;
>5/20/04
>
>Dear
Mr. Singeltary,
>
>The Western blot tests on these animals were
completed in April of this
>year. That means that we can begin the mouse
inoculations. To get the
>results of the Western blot tests, you will need
to submit a Freedom of
>Information Act request through our FOIA office.
The FAX number there is
>301-734-5941.
>
>Have a nice
day,
>
>Jim Rogers
>APHIS LPA
>
and with my
previous attempts for information via the FOIA through
this administration
(as you are probably very well aware of) they have
all been ignored/refused.
so any further attempts would be fruitless i am
sure.
thanks
anyway...
kindest
regards,
Terry
LAVET22@aol.com wrote:
> Mr.
Singeltary.
snip...
TSS
Terry S.
Singeltary Sr. wrote:
>
######## Bovine Spongiform Encephalopathy
> #########
>
>
Greetings Dr. Detwiler,
>
> glad to see you are still with us, you
had become very silent lately.
> hope you are enjoying semi
retirement.
>
> recently, i inquired through the BSE-L and via USDA official
about
> those Vermont sheep via belgium which there was an
Extraordinary
> Declaration of Emergency declared here in the USA due
to
> atypical scrapie. The thread is;
>
> Confiscation of
Sheep in Vermont and testing results ? Thu, 20 May 2004
> 12:10:03 -0500
"Terry S. Singeltary Sr." Bovine
> Spongiform Encephalopathy
BSE-L
>
>
>
>> Imported
>>
Belgium/Netherlands
>> Sheep Test Results
>>
Background
>> Factsheet
>> Veterinary Services April
2002
>> APHIS
>
>
>
>
snip...
>
>> Additional tests will be conducted to
determine
>> exactly what TSE the animals have BSE or
scrapie.
>> These tests involve the use of bioassays that
consist
>> of injecting mice with tissue from the infected
animals
>> and waiting for them to develop disease. This
testing
>> may take at least 2 to 3 years to
complete.
>
>
>
> http://www.aphis.usda.gov/lpa/pubs/fsheet_faq_notice/fs_ahvtsheeptr.pdf
>
> DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL
T.S.E.
> (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED
STATES
>
> http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&docid=fr20jy00-32
>
>
>
DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
> (PRION DISEASE) OF
FOREIGN ORIGIN IN THE UNITED STATES [2]
>
> http://frwebgate.access.gpo.gov/cgi-bin/getdoc.cgi?dbname=2000_register&docid=fr20jy00-31
>
>
>
or if those old urls dont work, go here;
>
> DECLARATION OF
EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
> (PRION
DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
> - Terry S. Singeltary
Sr. 7/20/00 (0)
>
>
> I was told that
;
>
>
> -------- Original Message --------
> Subject:
Re: hello Dr. Sutton...question please...scrapie...TSS
> Date: Thu, 20 May
2004 14:36:09 -0400
> From: Jim.D.Rogers@aphis.usda.gov
> To:
flounder@wt.net
>
>
>
> Dear Mr.
Singeltary,
>
> The Western blot tests on these animals were
completed in April of this
> year. That means that we can begin the mouse
inoculations. To get the
> results of the Western blot tests, you will
need to submit a Freedom of
> Information Act request through our FOIA
office. The FAX number there is
> 301-734-5941.
>
> Have a
nice day,
>
> Jim Rogers
> APHIS LPA
>
=========
>
>
> Dr. Detwiler, my question is, why have these
very important test been
> delayed for so long when we were told they were
to have been started
> some 2+ years ago?
>
> who made this
call to delay these very important test and why ?
>
> thank
you,
> with kindest regards,
>
> Terry
>
>
>
Linda Detwiler wrote:
>
>> ######## Bovine Spongiform
Encephalopathy
>> #########
>>
>> I m responding to
Roland's post about my quote in the article by Steve
>> Mitchell. I
spent a fair amount of time on the phone with Mr.
>> Mitchell on more
than
>> one occasion. The quote was one aspect of our conversation.
Even
>> the quote
>> included "probably". I explained about
proper location and sampling
>> condition of the brain. I also added in
our conversation that the
>> best methodology is
>> to utilize
both a test for PrP as well as histopathology when examining
>> brains
from cattle with CNS disease. This is why as early as
>> 1993-94 the
USDA
>> began using IHC in its TSE testing regime at the National
Veterinary
>> Services
>> Laboratory. However, utilizing only
a PrP test eliminates the
>> possibility of
>> diagnosing
another neurologic disease.
>>
>> All of the tests have
advantages and disadvantages. For example, I
>> have been
>>
to a number of laboratories in Europe and watched as technicians
took
>> the
>> test samples from the brain stem. They sample
hundreds per night.
>> If they get
>> distracted the may take
the sample lateral, or rostral to the
>> obex. If this
>>
animal was in an earlier stage of disease, there may be a very small
>>
amount of
>> PrPsc and limited to a single location in the brain. If
that sample
>> missed
>> the obex, the sample would be
negative and life goes on. With any
>> test using
>> a
homogenate I am not aware that there is any cross check for
location.
>>
>> With IHC, the pathologist can determine
location, however it too has
>> drawbacks in regard to sample
condition. There is no perfect test.
>> There are
>>
limitations to the tests themselves and there are limitations to all
>>
of the aspects
>> of collection. Hence utilizing multiple tests
especially for CNS
>> cases is
>> prudent. It is also prudent
to examine other locations of the brain
>> in the event
>> a
disease changes or something new emerges.
>>
>>
>>
Linda Detwiler
>>
>> #########
http://mailhost-alt.rz.uni-karlsruhe.de/warc/bse-l.html
>>
##########
>>
>>
>>
>
> #########
http://mailhost-alt.rz.uni-karlsruhe.de/warc/bse-l.html
>
##########
>
#########
http://mailhost-alt.rz.uni-karlsruhe.de/warc/bse-l.html
##########
Saturday, February 27, 2010
*** FINAL REPORT OF THE TESTING OF THE BELGIAN (VERMONT) SHEEP
February 27, 2010 IN SHORT ;
August 15, 2000
OIG case # NY-3399-56 REDACTED, VT
''Enclosed is OIG's notification that they have scheduled an investigation
of the following individual. REDACTED is alleged to have provided possibly
inaccurate test results involving diseased sheep. However, because the results
were determined to be inconclusive, no actual violation was actually
committed.''
snip...
PLEASE SEE FULL TEXT HERE ;
> > Date: Thu, 20 May 2004 14:36:09 –0400
> > > snip...
> > > FULL TEXT AND THREAD BETWEEN TSS, MAFF, USDA AND DR.
DETWILER HERE ;
OpenDocument
7. WHY is it that the Farm of the Mad Sheep of Mad River Valley were
quarantined for 5 years, but none of these farms from Texas and Alabama with
Atypical TSE in the Bovine, they have not been quarantined for 5 years, why not,
with the real risk of BSE to sheep, whom is to say this was not BSE ?
SOME DISTURBING TSE DATA FROM BELGIUM ;
Increased incidence of sporadic Creutzfeldt-Jakob disease in the age groups
between 70 and 90 years in Belgium
B. Van Everbroeck1, A. Michotte2, R. Sciot3, C. Godfraind4, M. Deprez5, S.
Quoilin6, J. -J. Martin1 and P. Cras1, 7
(1) Born-Bunge Institute (BBI), University of Antwerp (UA), Campus Drie
Eiken (CDE), Antwerp, Belgium
(2) Department of Neuropathology, Academic hospital, Free University of
Brussels, Brussels, Belgium
Page 17 of 98
8/3/2006
(3) Department of Pathology, Catholic University of Leuven, Leuven,
Belgium
(4) Pathology Laboratory, Catholic University of Louvain, Brussels,
Belgium
(5) Laboratory of Neuropathology, University of Liège, Sart Tilman, Liège,
Belgium
(6) Institute of Public Health-Louis Pasteur, Brussels, Belgium
(7) Laboratory of Neurobiology, BBI, UA, CDE, Universiteitsplein 1, B-2610
Wilrijk, Belgium Received: 28 October 2005 Accepted: 28 March 2006 Published
online: 12 July 2006 Abstract From 1998 a prospective surveillance study of
Creutzfeldt-Jakob disease (CJD) has been initiated in Belgium. In addition to
epidemiological data, information on cerebrospinal fluid biomarkers, prion
protein gene and brain neuropathology was collected. From 1-1-1998 to
31-12-2004, 188 patients were referred to the surveillance system. In 85
patients a ‘definite’ diagnosis of sporadic CJD (sCJD) could be made, whereas 26
patients remained ‘probable’. We further identified two unrelated patients with
an E200K mutation, and two patients with a seven octapeptide repeat insertion in
one family. In one patient a familial history was noted but genetic analysis was
not performed. In 72 patients different final diagnoses were made, Alzheimer’s
disease being the most frequent (N = 20). The demographic parameters of the
Belgian population were similar to those observed in the rest of Europe. We did
notice a significantly increased age-specific incidence (‰>‰6/106/year) of
sCJD patients between 70 and 90 years old in the period 2002– 2004 compared to
1998–2001 and retrospectively obtained data (1990–1997, p < 0.01). We
undertook a detailed clinical and biochemical analysis to investigate this
increase but could not identify any reason other than an increased vigilance for
the diagnosis.
In conclusion, our study identified that in the past sCJD may have been
underestimated in patients over age 70 although these patients are both
clinically and neurobiochemically similar to the general sCJD phenotype.
Keywords Diagnosis - Epidemiology - Prion disease - Transmissible spongiform
encephalopathy
BASE in cattle in Italy of Identification of a second bovine amyloidotic
spongiform encephalopathy: Molecular similarities with sporadic
Creutzfeldt-Jakob disease
Atypical Case of Bovine Spongiform Encephalopathy in an East-Flemish Cow in
Belgium
H. De Bosschere, DVM, PhD
S. Roels, DVM, PhD
E. Vanopdenbosch, DVM, Lic Page 18 of 98
8/3/2006
Veterinary and Agrochemical Research Centre (CODA/CERVA) National Reference
Laboratorium for Veterinary TSEs
Groeselenberg 99, B-1180
Ukkel (Brussels), Belgium
KEY WORDS: Bovine spongiform encephalopathy, BSE, Western blot, atypical
BSE.
ABSTRACT
For many years, researchers believed that only one bovine spongiform
encephalopathy (BSE) strain existed, in contrast to the many different scrapie
strains found. However, only very recently reports emerged about unconventional
BSE strains seen in Italy, France, and Japan. The present case describes an
atypical strain of BSE in Belgium in a 64-month old East-Flemish cow with an
electrophoretic profile and other features similar to those described in
Japan.
INTRODUCTION
Transmissible spongiform encephalopathies (TSEs), or prion diseases, are a
group of fatal neurodegenerative diseases including sheep and goat scrapie,
bovine spongiform encephalopathy (BSE), and Creutzfeldt-Jakob disease (CJD) in
humans. They are characterized by the accumulation of an abnormal protein,
called PrPsc, which is formed post translationally from the normal isoform
(PrPc).1,2 At present, the agent causing TSEs is still incompletely
characterized, although PrPsc is believed to be its major if not unique
constituent.3
Research in mice showed the existence of different scrapie strains.4,5
Scrapie strain discrimination is currently based on biologic typing in a panel
of inbred mice, using incubation time and brain pathology scoring as criteria.6
However, no large-scale studies of the molecular features of PrPsc have been
reported for bovine BSE to date. Till now, the BSE strain seemed to maintain
constant biologic and molecular properties even after experimental or accidental
passages into different species, such as mice, humans, primates, and sheep.7–10
However, very recently, variant forms of BSE have been reported in Japan, Italy,
and France.11-13 These forms were characterized by atypical histopathologic,
immunohistochemical, or biochemical phenotypes. The present case is the
description of the first atypical BSE case in Belgium.
MATERIALS AND METHODS
Since January 2001, all cattle older than 30 months are tested for TSE via
a rapid test (TeSeE-kit, Bio-Rad, Nazareth, Belgium) after EC regulation
999/2001.14,15 Samples positive according to the enzyme-linked immunosorbent
assay (ELISA) screening are further subjected to scrapie-associated fibrils
(SAF), histopathology, immunohistochemistry, and Western blot (WB) testing 16,17
at the National Reference Laboratory (NRL).
RESULTS
A positive ELISA sample from a 64-month-old East-Flemish cow or Belgian
white and red (Figure 1) was presented at the NRL for confirmation. The animal
was reported healthy before slaughter. The optical density (OD) titers at the
local laboratory were 2.324 and 2.116.16 The OD titers at the NRL were 0.953 and
0.708 (sample taken at the contralateral side of the first sampling side of the
obex region). The histopathology of the obex, pons, and midbrain
Page 19 of 98
8/3/2006
showed no spongiform changes; immunohistochemistry of the brainstem
revealed no signal of PrPsc accumulation typical for BSE; and SAF was negative.
However, WB analysis (Bovine WB, Bio-Rad, France; antibodies 12F10 and SAF60) of
the same homogenate that was prepared from the obex region for ELISA revealed a
small amount of PrPsc with an electrophoretic profile different from that of
typical BSE-associated PrPsc.18,19 The band on the gel of the non-glycosylated
form of PrPsc of the present case clearly showed a lower migration pattern
compared with that of a typical BSE case (Figure 2).
DISCUSSION
For many years, researchers assumed that only one BSE strain existed.7–10
Only in the past months, reports of atypical BSE cases were announced.11–13 The
Japanese case11 describes a very young bull (23 months) characterized by the
absence of spongiform changes and PrPsc deposits immunohistochemically. The WB
analysis revealed an electrophoretic profile different from that of typical BSE,
characterized by low content of the di-glycosylated molecular form of PrPsc and
a faster migration of the nonglycosylated form of PrPsc. In Italy,12 two BSE
affected cattle with a previously unrecognized neuropathologic profile and PrPsc
type were seen. These cases were determined using a different staining pattern
on immunohistochemistry, a difference in size and glycoform ratio of PrPsc on
immunoblot and a difference in regional distribution of lesions. The two cases
in France13 showed variant molecular features with a different PrPsc
electrophoretic profile from other BSE cases, mainly characterized by a higher
molecular mass of the nonglycosylated PrPsc. The present case shows the most
similarities (ie, identical electrophoretic profile, only ELISA and WB positive
and histopathology and immunohistochemistry negative) with the Japanese case,11
although the cow in the Japanese case was only 23 months old, and the cow in
this case was 64 months old.
The fact that these strains were detected worldwide and in several breeds
suggest that there is no local or breed dependent feature involved. It could be
that the WB techniques have become more specific within the past year in the
detection of minor differences in di-, mono-, and nonglycosylated molecular
forms of PrPsc. Infection of cattle by scrapie could also be considered since
scrapie can be transmitted by direct contact between animals or through
environmental contamination.13
In conclusion, this Belgian case should be added to the list of atypical
BSE strains only very recently detected worldwide and may contribute to further
research studies about epidemiologic significance. Current continued research on
BSE would appear to reveal different BSE strains in analogy with the different
scrapie strains.
ACKNOWLEDGMENTS
The authors wish to thank Rita Geeroms, Patrick Van Muylem, Stephanie
Durand, Raphaël Foubert and Amina Chama for their technical assistance. Mario
Vanpoucke is acknowledged for providing references.
REFERENCES
snip...
[PDF]Response to Public Comments - USDA Food Safety and ...
www.fsis.usda.gov/.../BSE_Risk_Asse... Cached
Food Safety and Inspection Service Loading...
Oct 31, 2005 - documents sent to the docket. ...
2 Expanded “Mad Cow” Safeguards Announced to Strengthen Existing ...
FSIS and APHIS that the estimated percentage of poultry litter fed to
..... 0579-AB93, Federal Register, Vol.70 No. .....
Comment #7: WHY is it that the Farm of the Mad Sheep of Mad River Valley
were.
Comment #6: WHAT happened to the test results and MOUSE BIO-ASSAYS of those
imported sheep from Belgium that were confiscated and slaughtered from the
Faillace's, what sort of TSE did these animals have?
Response: It is not clear how the test results referred to in this comment
are relevant to the Harvard BSE Risk Assessment Update. Sheep were not
considered in the risk assessment.
Comment #7: WHY is it that the Farm of the Mad Sheep of Mad River Valley
were quarantined for 5 years, but none of these farms from Texas and Alabama
with Atypical TSE in the Bovine, they have not been quarantined for 5 years, why
not, with the real risk of BSE to sheep, whom is to say this was not BSE ?
Response: This comment pertains to policy. As such, it is not addressed
here.
[PDF]Owens, Julie Page 1 of 98 8/3/2006 - USDA Food Safety ...
www.fsis.usda.gov/.../2006-0011-1.p... Cached
Food Safety and Inspection Service Loading... by F Greetings - 2006 -
Related articles Aug 3, 2006 -
Subject: [Docket No. FSIS-2006-0011] ... [Federal Register: July 12, 2006
(Volume 71, Number 133)]. [Notices] ..... group as compared to prior years, we
found that conflicting APHIS instructions .....
WHY is it that the Farm of the Mad Sheep of Mad River Valley were
quarantined for 5 years, but none of these.
> >> Imported
> >> Belgium/Netherlands
> >> Sheep Test Results
> >> Background
> >> Factsheet
> >> Veterinary Services April 2002
> >> APHIS
> > > >
> > > > snip...
> > > >> Additional tests will be conducted to determine
> >> exactly what TSE the animals have BSE or scrapie.
> >> These tests involve the use of bioassays that consist
> >> of injecting mice with tissue from the infected animals
> >> and waiting for them to develop disease. This testing
> >> may take at least 2 to 3 years to complete.
> > > > > > DECLARATION OF EXTRAORDINARY EMERGENCY
BECAUSE OF AN ATYPICAL T.S.E.
> > (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
> > > > > > > > > DECLARATION OF
EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E
> > (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [2]
> > > > > > > > > or if those old urls dont
work, go here;
> > > > DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN
ATYPICAL T.S.E
> > (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES
> > - Terry S.
> > Singeltary Sr. 7/20/00 (0)
> > > > > [Federal Register: July 20, 2000 (Volume 65,
Number 140)] [Notices]
> > [Page 45018]
>From the Federal Register Online via GPO Access
> > [wais.access.gpo.gov] [DOCID:fr20jy00-32]
> > > >
-----------------------------------------------------------------------
> > > > DEPARTMENT OF AGRICULTURE
> > > > Office of the Secretary
> > > > [Docket No. 00-072-1]
> > > > Declaration of Extraordinary Emergency Because of an
Atypical
> > Transmissible Spongiform Encephalopathy (Prion Disease) of
Foreign Origin
> > > > A transmissible spongiform encephalopathy (TSE)
(prion disease) of
> > foreign origin has been detected in the United States. It is
different
> > from TSE's previously diagnosed in the United States. The TSE
was
> > detected in the progeny of imported sheep. The imported sheep
and
> > their progeny are under quarantine in Vermont. Transmissible
> > spongiform encephalopathies are degenerative fatal diseases
that can
> > affect livestock. TSE's are caused by similar, as yet
uncharacterized,
> > agents that usually produce spongiform changes in the brain.
> > Post-mortem analysis has indicated positive results for an
atypical
> > TSE of foreign origin in four sheep in Vermont. Because of the
> > potentially serious consequences of allowing the disease to
spread to
> > other livestock in the United States, it is necessary to seize
and
> > dispose of those flocks of sheep in Vermont that are affected
with or
> > exposed to the disease, and their germ plasm. The existence of
the
> > atypical TSE of foreign origin represents a threat to U.S.
livestock.
> > It constitutes a real danger to the national economy and a
potential
> > serious burden on interstate and foreign commerce. The
Department has
> > reviewed the measures being taken by Vermont to quarantine and
> > regulate the flocks in question and has consulted with
appropriate
> > officials in the State of Vermont. Based on such review and
> > consultation, the Department has determined that Vermont does
not have
> > the funds to compensate flock owners for the seizure and
disposal of
> > flocks affected with or exposed to the disease, and their germ
plasm.
> > Without such funds, it will be unlikely to achieve expeditious
> > disposal of the flocks and germ plasm. Therefore, the
Department has
> > determined that an extraordinary emergency exists because of
the
> > existence of the atypical TSE in Vermont. This declaration of
> > extraordinary emergency authorizes the Secretary to seize,
quarantine,
> > and dispose of, in such manner as he deems necessary, any
animals that
> > he finds are affected with or exposed to the disease in
question, and
> > their germ plasm, and otherwise to carry out the provisions
and
> > purposes of the Act of July 2, 1962 (21 U.S.C. 134-134h). The
State of
> > Vermont has been informed of these facts.
> > > > Dated: This declaration of extraordinary emergency
shall become
> > effective July 14, 2000. Dan Glickman, Secretary of
Agriculture. [FR
> > Doc. 00-18367 Filed 7-19-00; 8:45 am] BILLING CODE 3410-34-P
THE
REST IS HISTORY, around 2005, what they were trying to stop at the Faillaces
front door, was then finally documented anyway, and since then, the Nor-98
‘foreign animal disease’ Winking smile , has spread from coast to coast in North
America.
***
The discovery of previously unrecognized prion diseases in both humans and
animals (i.e., Nor98 in small ruminants) demonstrates that the range of prion
diseases might be wider than expected and raises crucial questions about the
epidemiology and strain properties of these new forms. We are investigating this
latter issue by molecular and biological comparison of VPSPr, GSS and Nor98.
Saturday, July 6, 2013
Small Ruminant Nor98 Prions Share Biochemical Features with Human
Gerstmann-Sträussler-Scheinker Disease and Variably Protease-Sensitive
Prionopathy
Research Article
From: TSS
(216-119-138-157.ipset18.wt.net)
Subject: Re: More on
the Mad Sheep from Mad River Valley
Date: August 12, 2000 at
8:44 am PST
In Reply to: More on
the Mad Sheep from Mad River Valley posted by Terry S. Singeltary Sr. on
August 12, 2000 at 8:42 am:
######### Bovine Spongiform Encephalopathy #########
>BSE-suspected Vermont Sheep Get
Another
>Reprieve, USDA Backs Off Slaughter
Order
>
>BURLINGTON, Vt., August 10 (United Press International) --
Two
>flocks of exotic sheep scheduled for slaughter this week in
Vermont
>because of fears some of the animals might be infected with a
form
>of the deadly mad cow disease have won yet another
reprieve.
>
>The U.S. Department of Agriculture has temporarily
backed off plans
>to seize and destroy the animals, the Burlington Free
Press reported
>Thursday.
>
Actually something else happened in the
interim:
Vermont sheep still grazing as lawyers reach deal
Thu, Aug 10, 2000 Reuters
BURLINGTON, Vt., Aug 10 (Reuters) - Lawyers for owners of Vermont
sheep
suspected of having a neurological ailment similar to mad cow
disease
struck a deal with prosecutors on Thursday for a fuller court hearing
on
the animals' fate.
Under the agreement, lawyers for the farmers will forgo
afederal
appeals case in return for a more detailed hearing
Under the
agreement, lawyers for the farmers will forgo afederal
appeals case in return
for a more detailed hearing in a lower court, said
Tom Amidon, who represents
sheep farmer Houghton Freeman.
"So the sheep are on the farm awhile," Amidon
said. He hoped a new
hearing would allow for closer examination of claims by
the U.S.
Agriculture Department, which wants to seize and eliminate the 350
sheep
under scrutiny as a health precaution.
U.S. District Judge Garvan Murtha ruled last week the
Agriculture
Department could carry out its plan. But the farmers planned to
appeal
before Thursday's agreement changed the legal picture.
"We will
drop our appeal to the second circuit and in return the U.S.
attorneys office
in Vermont will allow us to hold a fuller court hearing,"
Amidon said.
Four sheep on Vermont farms near Warren, Vermont, tested positive
last
month for a disease known as TSE or transmissible
spongiform
encephalopathy, according to USDA officials.
TSE can cause scrapie, a fatal disease in sheep. It is also part of
a
family of diseases that includes deadly BSE, also known as
bovine
spongiform encephalopathy or mad cow disease.
No cases of BSE or mad cow disease have ever been found in the
United
States, but the human form of the disease is blamed for 75 deaths
in
Britain during the 1980s.
Breeding pairs for the two flocks of diary sheep came from Belgium
and
the Netherlands in the mid-90s.
Amidon said the Belgian government has sent a letter to the
USDA
requesting the return of all 350 sheep and that Freeman had already
lined
up a plane to transport them. Whether that will eventually occur
remains
############
http://mailhost.rz.uni-karlsruhe.de/warc/bse-l.html ############
BSE
INQUIRY DFAs
Sunday, May 18, 2008
BSE Inquiry DRAFT FACTUAL ACCOUNT DFA
BSE Inquiry DRAFT FACTUAL ACCOUNTS DFA's
Sunday, May 18, 2008
BSE, CJD, and Baby foods (the great debate 1999 to 2005)
Sunday, May 18, 2008
MAD COW DISEASE BSE CJD CHILDREN VACCINES
2015
SCIENTIFIC COMMITTEE OF THE BELGIAN FEDERAL AGENCY FOR THE SAFETY OF THE
FOOD CHAIN
Subject: RAPID ADVICE 17-2014 : Evaluation of the risk for public health
of casings in countries with a “negligible risk status for BSE” and on the risk
of modification of the list of specified risk materials (SRM) with regard to BSE
SCIENTIFIC COMMITTEE OF THE BELGIAN FEDERAL AGENCY FOR THE SAFETY OF THE
FOOD CHAIN
RAPID ADVICE 17-2014
Subject: Evaluation of the risk for public health of casings in
countries with a “negligible risk status for BSE” and on the risk of
modification of the list of specified risk materials (SRM) with regard to BSE
(Dossier SciCom 2014/22) Rapid advice approved by the Scientific Committee on
22nd October 2014.
Summary
The
Scientific Committee was asked to answer two questions in regard to a proposal
from the European Commission to no longer obligate Member States with a
negligible BSE risk status to remove and dispose the specified risk materials as
specified in Annex V to Regulation (EC) No 999/2001 of the European Parliament
and of the Council of 22 May 2001 laying down rules for the prevention, control
and eradication of certain transmissible spongiform encephalopathies. The aim of
this modification of the Regulation is to ensure that conditions for imports of
commodities from third countries are not more favorable than the conditions
applying to Member States with the same OIE BSE negligible risk status. More
specifically it was asked to the Scientific Committee:
-
If there is a difference in public health risk between the casings imported from
third countries with a “negligible risk status for BSE” and casings that come
from the 18 EU Member States with a “negligible risk status for BSE”?
-
If there is a significantly increased public health risk if, in the EU Member
States with a “negligible risk status for BSE”, the intestines are no longer
removed as SRM and if the other risk materials for BSE (the skull including the
brains and eyes, the spinal cord, the tonsils and the spine) are indeed
considered as SRM?
Due
to lack of availability of data on true prevalence and tissue infectivity of BSE
(classical as well as atypical BSE) the Scientific Committee was not able to
thoroughly investigate the questions.
Removal of specified risk materials from cattle at slaughter prevents
BSE infected materials from entering the human food chain.
The
Scientific Committee is of the opinion that, taking into consideration the
uncertainties in regard to the true prevalence of BSE (classical as well as
atypical BSE) in countries with a “negligible risk status for BSE” and given the
problems related with the early detection of asymptomatic BSE and given the
zoonotic significance of atypical BSE, that stopping with the routine removal of
specified risk materials during bovine slaughter will increase the risk for
public health.
2/14
The
Scientific Committee is not able to compare the public health risk of casings
from third countries and from the 18 EU Member States, both with a negligible
risk status for BSE, because of lack of data on true BSE prevalence and BSE
tissue infectivity (classical BSE and atypical BSE) in the considered
countries.
The
Scientific Committee is also not able to properly answer the second question if
there is a significantly increased public health risk if, in the EU Member
States with a “negligible risk status for BSE”, the intestines are no longer
removed as SRM due to lack of quantitative data on tissue infectivity of
different specified risk materials in slaughtered bovines in these countries.
There is also no information on tissue infectivity of atypical BSE cases. It is
known however that intestines are the portal of entry of prions and that they
are already infective before the prions reach the central nervous system.
The
final decision pertaining the need of removal of all or part of the specified
risk materials is a risk management decision and goes beyond the competencies of
the Scientific Committee.
Samenvatting Sneladvies over de risico’s voor de volksgezondheid van
worstenvellen in landen met een “verwaarloosbaar risicostatuut voor BSE” en over
de risico’s van wijziging van een lijst van gespecifieerde risicomaterialen
(GRM) voor BSE
snip...
In
conclusion the Scientific Committee is not able to answer this question with an
acceptable degree of uncertainty because of lack of data on true prevalence of
BSE (classical as well as atypical forms of BSE) in the considered countries. It
reiterates its concern regarding the import of certain animal products from
third countries with a ‘negligible BSE risk status’ as stated in rapid advice
SciCom 16-2013.
snip...
2.
Is there a significantly increased public health risk if, in the EU Member
States with a “negligible risk status for BSE”, the intestines are no longer
removed as SRM while the other risk materials for BSE (the skull including the
brains and eyes, the spinal cord, the tonsils and the spine) are indeed
considered as SRM?
Once again the Scientific Committee is not able to properly answer this
question because of lack of quantitative data on tissue infectivity of different
specified risk materials in slaughtered bovines in EU Member States with a
“negligible risk status for BSE”.
BSE
infected animals may enter undetected the food chain due to the low sensitivity
of the diagnostic tests. Further on the classical BSE agent accumulates from the
first months post exposure in particular segments of the bovine intestines and
persists till clinical onset. In addition no information is available about the
infectivity of tissues by the atypical BSE agent, especially in the
intestines.
If
intestines from cattle in EU Member States with a “negligible risk status for
BSE” are no longer removed as SRM and are allowed to enter the food chain the
public health risk will be increased. The degree of rise in risk level cannot be
determined. According to EFSA Journal 2014;12(2):3554, the TSEi model indicated
that the removal of the last four meters of the small intestine and of the
caecum from the food and feed chain would result in a major reduction of the
Classical BSE exposure risk associated with intestine and mesentery in
cattle.
Referring to its previous advice 16-2013 the Scientific Committee
repeats that stopping with the routine removal of all specified risk materials
during bovine slaughter will increase the risks of exposure of the population to
BSE because of the uncertainty related to the detection of BSE. This uncertainty
is the consequence of the long incubation period (especially in cases of
atypical BSE), the low sensitivity of the available diagnostic methods, the
apparent spontaneous nature of atypical BSE, the lack of a clear clinical
picture of atypical BSE cases and the reduction in number of tests in healthy
slaughtered animals.
The
final decision pertaining the need of removal of all or part of the specified
risk materials is a decision to be taken by the risk manager and goes beyond the
competencies of the Scientific Committee.
5.
Conclusion
Removal of specified risk materials from cattle at slaughter prevents
BSE infected materials from entering the human food chain.
The
Scientific Committee is of the opinion that, taking into consideration the
uncertainties in regard to the true prevalence of BSE (including classical as
well asaAtypical BSE) in countries with a “negligible risk status for BSE” and
given the problems related with the early detection of asymptomatic BSE and
given the zoonotic character of atypical BSE, stopping with the routine removal
of all specified risk materials during bovine slaughter will increase the risk
for public health.
12/14
The
Scientific Committee is not able to compare the public health risk of casings
from third countries and from the 18 EU Member States both with a negligible
risk status for BSE because of lack of data on true BSE prevalence (classical
BSE and atypical BSE) in the considered countries.
The
Scientific Committee is not able to properly answer the question if there is a
significantly increased public health risk if, in the EU Member States with a
“negligible risk status for BSE”, the intestines are no longer removed as SRM
due to lack of quantitative data on tissue infectivity of different specified
risk materials in slaughtered bovines in these countries. There is also no
information on tissue infectivity by the agent of atypical BSE.
On
behalf of the Scientific Committee, The President Prof. Dr. E. Thiry (Sgd.)
Brussels, 06/11/2014
References
snip...end
Thursday, July 24, 2014
Protocol for further laboratory investigations into the distribution of
infectivity of Atypical BSE SCIENTIFIC REPORT OF EFSA
P7.09
Biochemical screening for identification of atypical bse in belgium,
1999-present
Authors
Alexandre DobIy: Caroline Rodeghiero, Riet Geeroms; Stephanie Durand,
Jessica De Sloovere, Emanuel Yanopdenbosch, Stefan Roels,
Content
Background: Recently atypical forms of BSE have been described. Western
blot analyses showed that, in comparison to the classic BSE (C-type), they are
demonstrable by a higher or lower molecular weight of the unglycosylated PrPres.
They Viere thus named H-type and L-type BSE (L-type is also called BASE). In
addition they show a lower proportion of diglycosylated PrPres than C-type.
These emerging types represent different strains of BSE. They show unique
incubation periods and histological lesions. Such types have been described on
different continents. Indeed they might correspond to "sporadic" forms of BSE.
In 2004 we already described one L-type in Belgium.
Objective: We retrospectively analysed the bovines at least 7-year-old
in the Belgian archive of BSE diagnosed cattle in order to determine the
prevalence of the two types of atypical BSE in Belgium.
Methods: We analysed homogenates from 39 bovines of 93 months old in
median (min: 84, max: 181 months). The most recent one was diagnosed in 2006. We
used Western blot with a panel of anti-PrP antibodies (Ab). They detect
different regions of the PrP protein, from N-terminal to C-terminal: 12B2, 9A2,
Sha31. SAFB4, 94B4. Their combination is aimed at an efficient typing
diagnostic. We detected bound Ab with SuperSignal West Dura (Pierce) and
analysed PrPres, signals with an image-analysis software (Quantity One,
Bio-Rad).
Results: The results are still under analysis. We will detail the most
crucial characteristics for typing PrPres. These include 1) the apparent
molecular mass of the an-, mono- and diglycosylated bands, 2) the binding
affinity to the five Ab (e.g.12B2 for H-type), 3) the presence of a fourth
(unglycosylated) band and 4) the glycoprofile based on the relative proportions
of the visible bands.
Discussion: The emergence of atypical types of BSE is partially due to a
better knowledge of prion strains and more efficient diagnostic techniques. As
the area in the brain where the PrPres is deposited can differ drastically
between the types, it is essential to ascertain that the sampling techniques and
analyses are adapted to these new types. As these new strains seem more virulent
than classic types, they represent one of the next challenges in the field of
prions.
Atypical Case of Bovine Spongiform Encephalopathy in an East-Flemish Cow
in Belgium
H.
De Bosschere, DVM, PhD
S.
Roels, DVM, PhD
E.
Vanopdenbosch, DVM, Lic
Veterinary and Agrochemical Research Centre (CODA/CERVA)
National Reference Laboratorium for Veterinary TSEs
Groeselenberg 99, B-1180
Ukkel (Brussels), Belgium
KEY
WORDS: Bovine spongiform encephalopathy, BSE, Western blot, atypical BSE.
ABSTRACT
For
many years, researchers believed that only one bovine spongiform encephalopathy
(BSE) strain existed, in contrast to the many different scrapie strains found.
However, only very recently reports emerged about unconventional BSE strains
seen in Italy, France, and Japan. The present case describes an atypical strain
of BSE in Belgium in a 64-month-old East-Flemish cow with an electrophoretic
profile and other features similar to those described in Japan.
INTRODUCTION
Transmissible spongiform encephalopathies (TSEs), or prion diseases, are
a group of fatal neurodegenerative diseases including sheep and goat scrapie,
bovine spongiform encephalopathy (BSE), and Creutzfeldt-Jakob disease (CJD) in
humans. They are characterized by the accumulation of an abnormal protein,
called PrPsc, which is formed post-translationally from the normal isoform
(PrPc).1,2 At present, the agent causing TSEs is still incompletely
characterized, although PrPsc is believed to be its major if not unique
constituent.3
Research in mice showed the existence of different scrapie strains.4,5
Scrapie strain discrimination is currently based on biologic typing in a panel
of inbred mice, using incubation time and brain pathology scoring as criteria.6
However, no large-scale studies of the molecular features of PrPsc have been
reported for bovine BSE to date. Till now, the BSE strain seemed to maintain
constant biologic and molecular properties even after experimental or accidental
passages into different species, such as mice, humans, primates, and sheep.7-10
However, very recently, variant forms of BSE have been reported in Japan, Italy,
and France.11-13 These forms were characterized by atypical histopathologic,
immunohistochemical, or biochemical phenotypes. The present case is the
description of the first atypical BSE case in Belgium.
snip...
In
conclusion, this Belgian case should be added to the list of atypical BSE
strains only very recently detected worldwide and may contribute to further
research studies about epidemiologic significance. Current continued research on
BSE would appear to reveal different BSE strains in analogy with the different
scrapie strains.
-------- Original Message --------
Subject: Atypical Case of Bovine Spongiform Encephalopathy in an
East-Flemish Cow in Belgium
Date: Fri, 04 Feb 2005 10:59:33 –0600
From: "Terry S. Singeltary Sr."
To:
Bovine Spongiform Encephalopathy
CC:
cjdvoice@yahoogroups.com
Atypical Case of Bovine Spongiform Encephalopathy in an East-Flemish Cow
in Belgium
H.
De Bosschere, DVM, PhD
S.
Roels, DVM, PhD
E.
Vanopdenbosch, DVM, Lic
Veterinary and Agrochemical Research Centre (CODA/CERVA)
National Reference Laboratorium for Veterinary TSEs
Groeselenberg 99, B-1180
Ukkel (Brussels), Belgium
KEY
WORDS: Bovine spongiform encephalopathy, BSE, Western blot, atypical BSE.
ABSTRACT
For
many years, researchers believed that only one bovine spongiform encephalopathy
(BSE) strain existed, in contrast to the many different scrapie strains found.
However, only very recently reports emerged about unconventional BSE strains
seen in Italy, France, and Japan. The present case describes an atypical strain
of BSE in Belgium in a 64-month-old East-Flemish cow with an electrophoretic
profile and other features similar to those described in Japan.
INTRODUCTION
Transmissible spongiform encephalopathies (TSEs), or prion diseases, are
a group of fatal neurodegenerative diseases including sheep and goat scrapie,
bovine spongiform encephalopathy (BSE), and Creutzfeldt-Jakob disease (CJD) in
humans. They are characterized by the accumulation of an abnormal protein,
called PrPsc, which is formed post-translationally from the normal isoform
(PrPc).1,2 At present, the agent causing TSEs is still incompletely
characterized, although PrPsc is believed to be its major if not unique
constituent.3
Research in mice showed the existence of different scrapie strains.4,5
Scrapie strain discrimination is currently based on biologic typing in a panel
of inbred mice, using incubation time and brain pathology scoring as criteria.6
However, no large-scale studies of the molecular features of PrPsc have been
reported for bovine BSE to date. Till now, the BSE strain seemed to maintain
constant biologic and molecular properties even after experimental or accidental
passages into different species, such as mice, humans, primates, and sheep.7 10
However, very recently, variant forms of BSE have been reported in Japan, Italy,
and France.11-13 These forms were characterized by atypical histopathologic,
immunohistochemical, or biochemical phenotypes. The present case is the
description of the first atypical BSE case in Belgium.
MATERIALS AND METHODS
Since January 2001, all cattle older than 30 months are tested for TSE
via a rapid test (TeSeE-kit, Bio-Rad, Nazareth, Belgium) after EC regulation
999/2001.14,15 Samples positive according to the enzyme-linked immunosorbent
assay (ELISA) screening are further subjected to scrapie-associated fibrils
(SAF), histopathology, immunohistochemistry, and Western blot (WB) testing16,17
at the National Reference Laboratory (NRL).
RESULTS
A
positive ELISA sample from a 64-month-old East-Flemish cow or Belgian white and
red (Figure 1) was presented at the NRL for confirmation. The animal was
reported healthy before slaughter. The optical density (OD) titers at the local
laboratory were 2.324 and 2.116.16 The OD titers at the NRL were 0.953 and 0.708
(sample taken at the contralateral side of the first sampling side of the obex
region). The histopathology of the obex, pons, and midbrain showed no spongiform
changes; immunohistochemistry of the brainstem revealed no signal of PrPsc
accumulation typical for BSE; and SAF was negative. However, WB analysis (Bovine
WB, Bio-Rad, France; antibodies 12F10 and SAF60) of the same homogenate that was
prepared from the obex region for ELISA revealed a small amount of PrPsc with an
electrophoretic profile different from that of typical BSE-associated
PrPsc.18,19 The band on the gel of the non-glycosylated form of PrPsc of the
present case clearly showed a lower migration pattern compared with that of a
typical BSE case (Figure 2).
DISCUSSION
For
many years, researchers assumed that only one BSE strain existed.7 10 Only in
the past months, reports of atypical BSE cases were announced.11 13 The Japanese
case11 describes a very young bull (23 months) characterized by the absence of
spongiform changes and PrPsc deposits immunohistochemically. The WB analysis
revealed an electrophoretic profile different from that of typical BSE,
characterized by low content of the di-glycosylated molecular form of PrPsc and
a faster migration of the nonglycosylated form of PrPsc. In Italy,12 two BSE
affected cattle with a previously unrecognized neuropathologic profile and PrPsc
type were seen. These cases were determined using a different staining pattern
on immunohistochemistry, a difference in size and glycoform ratio of PrPsc on
immunoblot and a difference in regional distribution of lesions. The two cases
in France13 showed variant molecular features with a different PrPsc
electrophoretic profile from other BSE cases, mainly characterized by a higher
molecular mass of the nonglycosylated PrPsc. The present case shows the most
similarities (ie, identical electrophoretic profile, only ELISA and WB positive
and histopathology and immunohistochemistry negative) with the Japanese case,11
although the cow in the Japanese case was only 23 months old, and the cow in
this case was 64 months old.
The
fact that these strains were detected worldwide and in several breeds suggest
that there is no local or breed-dependent feature involved. It could be that the
WB techniques have become more specific within the past year in the detection of
minor differences in di-, mono-, and nonglycosylated molecular forms of PrPsc.
Infection of cattle by scrapie could also be considered since scrapie can be
transmitted by direct contact between animals or through environmental
contamination.13
In
conclusion, this Belgian case should be added to the list of atypical BSE
strains only very recently detected worldwide and may contribute to further
research studies about epidemiologic significance. Current continued research on
BSE would appear to reveal different BSE strains in analogy with the different
scrapie strains.
ACKNOWLEDGMENTS
The
authors wish to thank Rita Geeroms, Patrick Van Muylem, Stephanie Durand,
Raphaël Foubert and Amina Chama for their technical assistance. Mario Vanpoucke
is acknowledged for providing references.
REFERENCES
1.
Oesch B, Westaway D, Walchii M, et al: A cellular gene encodes PrP 27 30
protein. Cell 40:735 746, 1985.
2.
Prusiner SB, De Armond SJ: Prion diseases and neurodegeneration. Annu Rev
Neurosci 17:311 339, 1994.
3.
Prusiner SB: Scrapie prions. Annu Rev Microbiol 43:345 374, 1989.
4.
Bruce M, Dickinson AG: Biological evidence that scrapie agent has an independent
genome. J Gen Virol 68:79 89, 1987.
5.
Fraser H, Dickinson AG: Scrapie in mice: Agent strain differences in the
distribution and intensity of grey matter vacuolation. J Comp Pathol 83:29 40,
1973.
6.
Bruce M, McConnell I, Fraser H, Dickinson AG: The disease characteristics of
different strains of scrapie in Sinc Congenic mice lines: Impications for the
nature of the agent and host control of pathogenesis. J Virol 72:595 603, 1991.
7.
Bruce M, Chree A, McDonnell I, et al: Transmission of bovine spongiform
encephalopathy and scrapie to mice: Strain variation and the species barrier.
Philos Trans R Soc Lon Ser B 343:405 411, 1994.
8.
Bruce M, Will RG, Ironside JW, et al: Transmissions to mice indicate that new
variant CJD is caused by the BSE agent. Nature 389:498 501, 1997.
9.
Foster JD, Bruce M, McDonnell I, et al: Detection of BSE infectivity in brain
and spleen of experimentally infected sheep. Vet Rec 138:546 548, 1996.
10.
Lasmezas CI, Fournier J-G, Nouvel V, et al: Adaptation of the bovine spongiform
encephalopathy agent to primates and comparison with Creutzfeldt-Jakob disease:
Implications for human health. Proc Natl Acd Sci U S A 98:4142 4147, 2001.
11.
Yamakawa Y, Hagiwara K, Nohtomi K, et al, for the Expert Commitee for BSE
Diagnosis, Ministry of Health, Labour and Welfare of Japan: Atypical proteinase
K-resistant prion protein (PrPres) observed in an apparently healthy
23-month-old Holstein steer. Jpn J Infect Dis 56:221 222, 2003.
12.
Casalone C, Zanusso G, Acutis PL, et al: Identification of a novel molecular and
neuropathological BSE phenotype in Italy: International Conference on Prion
Disease: from basic research to intervention concepts. Gasreig, München, 8 10
October, 2003.
13.
Biacabe AG, Laplanche JL, Ryder S, Baron T: A molecular variant of bovine
spongiform encephalopathy. International Conference on Prion Disease: From basic
research to intervention concepts. Gasreig, München, 8 10 October, 2003.
14.
De Becker D, Roels S, Vanopdenbosch E: BSE onderzoek: opsporen van PrPres door
middel van de BIO-RAD Platelia BSE-kit. Vlaams Diergeneeskundig Tijdschrift
69:382 384, 2000.
15.
Roels S, Demeyer G, Tedik K, et al: Variance of mass (volume) taken with the
calibrated syringe and of the results provided by the Bio-Rad Platelia BSE test
upon storage of brainstem samples at 20°C. Anim Res 51:493 499, 2002.
16.
Roels S, De Bosschere H, Saegerman C, et al: BSE and scrapie testing in Belgium:
general overview. New Food: accepted, 2004.
17.
Vanopdenbosch E, Dechamps P, Dufey J, et al: Le premier cas d encephalopathie
spongioforme bovine diagnostique en Belgique. Annales de Médicine Vétérinaire
142:111 118, 1998.
18.
Collinge J, Sidle KCL, Meads J, et al: Molecular analysis of prion strain
variation and the aetiology of new variant CJD. Nature 383:685 690, 1996.
19.
Hill AF, Desbruslais M, Joiner S, et al: The same prion strain causes vCJD and
BSE. Nature 389:448 450, 1997.
Figure 1. Photograph of the East-Flemish cattle breed or the Belgian
white and red.
Figure 2. Bovine Western blot (Bio-Rad, France) using antibodies 12F10
and SAF60. MM, Magic mark; Atyp. BSE, Atypical BSE case (present case); Ref1,
Reference 1 of a classical BSE case; Ref2, Reference 2 of a classical BSE case.
The third band of the non-glycosylated PrPsc of the Atyp. BSE case (left
rectangle) shows a markedly faster migration compared to the Ref1 and Ref2 cases
(right rectangle).
P.6.- Atypical case of bovine spongiform encephalopathy in an
East-Flemish cow in Belgium.
H.
De Bosschere1, S. Roels2, E. Vanopdenbosch3
1
Veterinary and Agrochemical Research Centre (CODA / CERVA), National Reference
Laboratory for Veterinary TSE (Belgium & Luxemburg) , Unit Pathology,
Department of Biocontrol, Groeselenberg 99, B-1180 Brussels (Ukkel), Belgium,
(hedeb@var.fgov.be)
2
idem, (stroe@var.fgov.be)
3
idem, (emvan@var.fgov.be)
Bovine spongiform encephalopathy (BSE) is a prion disease with a fatal
neurodegenerative pathogenesis. It is characterized by the accumulation of an
abnormal protein (PrPres), formed posttranslationally from the normal isoform
(PrPc). Research in mice showed the existence of different sheep scrapie
strains. Scrapie strain discrimination is currently based upon biological typing
in a panel of inbred mice. However, no large scale studies of the molecular
features of PrPres have been reported for bovine BSE to date. Till now, the BSE
strain seemed to maintain constant biological and molecular properties even
after experimental or accidental passages into different species. Very recently,
variant forms of BSE have been reported in Japan, Italy and France. These forms
were characterized by atypical histopathological, immunohistochemical and/or
biochemical phenotype compared to the classical BSE strain. The present case
describes the first Belgian atypical BSE case. Since January 1st 2001, all
cattle older than 30 months is tested for TSE via a rapid test following EC
regulation 999/2001. Samples positive according to the ELISA screening are
further subjected to scrapie associated fibrils (SAF), histopathology,
immunohistochemistry and Western blot (WB) at the NRL. A positive ELISA sample
from a 64 month-old East-Flemish or Belgian white and red cow was presented at
the NRL for confirmation. The histopathology of the obex, pons and midbrain was
negative, immunohistochemistry and SAF were also negative. However, WB analysis
was positive with an electrophoretic profile different from that of a typical
BSE case. The band on the gel of the non-glycosylated form of PrPres of the
present case clearly showed a lower migratrion pattern compared to that of a
typical BSE case. For many years it was assumed that there was only one BSE
strain. Only very recently, reports of atypical BSE cases were announced in
Japan, Italy and France. The Japanese case describes a very young bull (23
months) negative on histopathology and immunohistochemistry and a WB
electrophoretic profile different from that of classical BSE. The Italians
observed two BSE affected cattle with a a different staining pattern on
immunohistochemistry, a difference in size and glycoform ratio of PrPres on WB
and a difference in regional distribution of lesions. The French two cases
showed variant molecular features with a different electrophoretic profile from
other BSE cases. The present case shows the most similarities with the Japanese
case (except for the age). The fact that these strains were detected worldwide
and in several breeds suggest that there is no local or breed dependent feature
involved. It could be that the WB techniques have become more specifique within
the last year or infection of cattle by scrapie could also be considered. In
conclusion, continued research on BSE reveals nowadays different BSE strains in
analogy with the different sheep scrapie strains. Atypical BSE cases may
question the significance and efficiency of the BSE epidemio-surveillance
protocol and the validation of the confirmatory tests.
Keywords
Bovine spongiform encephalopathy, BSE, Western Blot, atypical BSE
Report on the assessment of the Geographical BSE-risk of BELGIUM July
2000
-
42 -
5.
CONCLUSION ON THE GEOGRAPHICAL BSE RISK
5.1
The current GBR
The
current geographical BSE-risk (GBR) level is III, i.e. BSE is confirmed in
domestic cattle (last and only case in 1997) at a lower level. However, the
observed incidence of clinical cases over the last 12 months (1 March 1999 to 29
February 2000) was 2.7 per 1 Million adult cattle. This figure is generated by a
passive surveillance system that is not able to identify all clinical
cases.
5.2
The expected development of the GBR
Assuming that measures in place continue to be appropriately implemented and no
new external challenge occurs, the GBR is expected to decrease over time.
However, this does not exclude that cattle infected by the BSE-agent in the past
(before 1998/99) may be identified as clinical cases in the foreseeable
future.
5.3
Recommendations for influencing the future GBR
Good implementation of the bans should be ensured.
In addition, expanding the surveillance system to target asymptomatic cattle in
risk sub-populations such as adult fallen stock and adult cattle presented for
emergency slaughter will allow verification of the current GBR-assessment and
monitoring its future trend.
American Association of Zoo Veterinarians Infectious Disease Committee
Manual 2013
BOVINE SPONGIFORM ENCEPHALOPATHY (BSE)
Little is known about atypical BSE. The origin and natural routes of
transmission, if any, have yet to be determined. Almost all cases have been in
older cattle (usually > 8 years of age) that have shown little resemblance to
the clinic-pathological picture seen in classical disease. It has been suggested
that the disease may be sporadic or be caused by a genetic mutation, but no
convincing evidence has been found to support either of these ideas. The correct
answer will probably only come by study of the future annual incidence curves of
both types of disease. Regardless of the origin of atypical BSE, the possibility
of recycling the disease in cattle and other ruminants, as well as the potential
for transmission to humans, mandate a continuation of feed and specified-risk
materials (SRM) bans, together with diagnostic testing programs for some time to
come.
snip...
Naturally occurring cases of BSE in species other than cattle have been
very limited and have been linked to exposure to contaminated feed or infected
carcasses. The majority of cases originated in the UK and like BSE in cattle,
have declined with the implementation of feed controls. None of the exotic
animals were infected in the wild.
Experts who may be consulted: Linda A. Detwiler, DVM Clinical Professor
Department of Pathobiology and Population Medicine
College of Veterinary Medicine Mississippi State University 732-580-9391
Fax: 732-741-7751 ldetwiler@belle-terre.com
Published in : European Journal of Epidemiology (2006), vol. 21, pp.
443-447
Status : Postprint (Author’s version)
Increased incidence of sporadic Creutzfeldt-Jakob disease in the age
groups between 70 and 90 years in Belgium
B.
Van Everbroeck1, A. Michotte2, R. Sciot3, C. Godfraind4, M. Deprez5, S.
Quoilin6, J.-J. Martin1 & P. Cras1 1Born-Bunge Institute (BBI), University
of Antwerp (UA), Campus Drie Eiken (CDE), Antwerp, Belgium; 2Department of
Neuropathology, Academic hospital, Free University of Brussels, Brussels,
Belgium; 3Department of Pathology, Catholic University of Leuven, Leuven,
Belgium; 4Pathology Laboratory, Catholic University of Louvain, Brussels,
Belgium; 5Laboratory of Neuropathology, University of Liège, Sart Tilman, Liège,
Belgium; 6Institute of Public Health-Louis Pasteur, Brussels, Belgium
Abstract
From 1998 a prospective surveillance study of Creutzfeldt-Jakob disease
(CJD) has been initiated in Belgium. In addition to epidemiological data,
information on cerebrospinal fluid biomarkers, prion protein gene and brain
neuropathology was collected. From 1-1-1998 to 31-12-2004, 188 patients were
referred to the surveillance system. In 85 patients a 'definite' diagnosis of
sporadic CJD (sCJD) could be made, whereas 26 patients remained 'probable'. We
further identified two unrelated patients with an E200K mutation, and two
patients with a seven octapeptide repeat insertion in one family. In one patient
a familial history was noted but genetic analysis was not performed. In 72
patients different final diagnoses were made, Alzheimer's disease being the most
frequent (N = 20). The demographic parameters of the Belgian population were
similar to those observed in the rest of Europe. We did notice a significantly
increased age-specific incidence (>6/106/ year) of sCJD patients between 70
and 90 years old in the period 2002-2004 compared to 1998-2001 and
retrospectively obtained data (1990-1997, p < 0.01). We undertook a detailed
clinical and biochemical analysis to investigate this increase but could not
identify any reason other than an increased vigilance for the diagnosis. In
conclusion, our study identified that in the past sCJD may have been
underestimated in patients over age 70 although these patients are both
clinically and neurobiochemically similar to the general sCJD phenotype.
Keywords : Diagnosis ; Epidemiology ; Prion disease ; Transmissible
spongiform encephalopathy
To
date, 27 cases of L-BSE and 24 cases of H-BSE have been reported worldwide
(16), thus meaning that the prevalence of atypical BSE is considerably lower
than that of C-BSE. However, recent studies showed that L-BSE is easily
transmissible to transgenic mice expressing human (17,18) or bovine (19,20)
prion protein, as well as to non-human primates (21), with shorter incubation
periods than for the transmission of C-BSE to these animals.
***The virulent nature of L-BSE has stimulated new concern for human
public health since the transmission of C-BSE to humans resulted in variant
Creutzfeldt-Jakob disease (vCJD) (4-7), a new emergent prion disease.
***Infectivity in skeletal muscle of BASE-infected cattle
***The present data offer novel information on the tropism of the BASE
agent and highlight relevant public health issues. While the transmission
barrier for classical BSE is high in most species, BASE prions are readily
transmissible to a variety of mammals including non-human primates [11–13,35].
Accordingly, the possibility of spreading of BASE prions through skeletal muscle
to other species should be taken into account and evaluated in risk analysis
studies.
P.4.23
Transmission of atypical BSE in humanized mouse models
Liuting Qing1, Wenquan Zou1, Cristina Casalone2, Martin Groschup3,
Miroslaw Polak4, Maria Caramelli2, Pierluigi Gambetti1, Juergen Richt5,
Qingzhong Kong1 1Case Western Reserve University, USA; 2Instituto
Zooprofilattico Sperimentale, Italy; 3Friedrich-Loeffler-Institut, Germany;
4National Veterinary Research Institute, Poland; 5Kansas State University
(Previously at USDA National Animal Disease Center), USA
Background: Classical BSE is a world-wide prion disease in cattle, and
the classical BSE strain (BSE-C) has led to over 200 cases of clinical human
infection (variant CJD). Atypical BSE cases have been discovered in three
continents since 2004; they include the L-type (also named BASE), the H-type,
and the first reported case of naturally occurring BSE with mutated bovine PRNP
(termed BSE-M). The public health risks posed by atypical BSE were largely
undefined.
Objectives: To investigate these atypical BSE types in terms of their
transmissibility and phenotypes in humanized mice. Methods: Transgenic mice
expressing human PrP were inoculated with several classical (C-type) and
atypical (L-, H-, or Mtype) BSE isolates, and the transmission rate, incubation
time, characteristics and distribution of PrPSc, symptoms, and histopathology
were or will be examined and compared.
Results: Sixty percent of BASE-inoculated humanized mice became infected
with minimal spongiosis and an average incubation time of 20-22 months, whereas
only one of the C-type BSE-inoculated mice developed prion disease after more
than 2 years. Protease-resistant PrPSc in BASE-infected humanized Tg mouse
brains was biochemically different from bovine BASE or sCJD. PrPSc was also
detected in the spleen of 22% of BASE-infected humanized mice, but not in those
infected with sCJD. Secondary transmission of BASE in the humanized mice led to
a small reduction in incubation time. The atypical BSE-H strain is also
transmissible with distinct phenotypes in the humanized mice, but no BSE-M
transmission has been observed so far.
Discussion: Our results demonstrate that BASE is more virulent than
classical BSE, has a lymphotropic phenotype, and displays a modest transmission
barrier in our humanized mice. BSE-H is also transmissible in our humanized Tg
mice. The possibility of more than two atypical BSE strains will be
discussed.
Supported by NINDS NS052319, NIA AG14359, and NIH AI 77774.
2014
***Moreover, L-BSE has been transmitted more easily to transgenic mice
overexpressing a human PrP [13,14] or to primates [15,16] than C-BSE.
***It has been suggested that some sporadic CJD subtypes in humans may
result from an exposure to the L-BSE agent.
***
Lending support to this hypothesis, pathological and biochemical similarities
have been observed between L-BSE and an sCJD subtype (MV genotype at codon 129
of PRNP) [17], and between L-BSE infected non-human primate and another sCJD
subtype (MM genotype) [15].
snip...
Monday, October 10, 2011
EFSA Journal 2011 The European Response to BSE: A Success Story
snip...
EFSA and the European Centre for Disease Prevention and Control (ECDC)
recently delivered a scientific opinion on any possible epidemiological or
molecular association between TSEs in animals and humans (EFSA Panel on
Biological Hazards (BIOHAZ) and ECDC, 2011). This opinion confirmed Classical
BSE prions as the only TSE agents demonstrated to be zoonotic so far
***
but the possibility that a small proportion of human cases so far classified as
"sporadic" CJD are of zoonotic origin could not be excluded.
***
Moreover, transmission experiments to non-human primates suggest that some TSE
agents in addition to Classical BSE prions in cattle (namely L-type Atypical
BSE, Classical BSE in sheep, transmissible mink encephalopathy (TME) and chronic
wasting disease (CWD) agents) might have zoonotic potential.
snip...
Thursday, August 12, 2010
Seven main threats for the future linked to prions
First threat
The
TSE road map defining the evolution of European policy for protection against
prion diseases is based on a certain numbers of hypotheses some of which may
turn out to be erroneous. In particular, a form of BSE (called atypical Bovine
Spongiform Encephalopathy), recently identified by systematic testing in aged
cattle without clinical signs, may be the origin of classical BSE and thus
potentially constitute a reservoir, which may be impossible to eradicate if a
sporadic origin is confirmed.
***
Also, a link is suspected between atypical BSE and some apparently sporadic
cases of Creutzfeldt-Jakob disease in humans.
***
These atypical BSE cases constitute an unforeseen first threat that could
sharply modify the European approach to prion diseases.
Second threat
snip...
snip...see more here ;
Saturday, January 31, 2015
RAPID ADVICE 17-2014 : Evaluation of the risk for public health of
casings in countries with a “negligible risk status for BSE” and on the risk of
modification of the list of specified risk materials (SRM) with regard to BSE
Tuesday, December 16, 2014
***
Evidence for zoonotic potential of ovine scrapie prions
Hervé Cassard,1, n1 Juan-Maria Torres,2, n1 Caroline Lacroux,1,
Jean-Yves Douet,1, Sylvie L. Benestad,3, Frédéric Lantier,4, Séverine Lugan,1,
Isabelle Lantier,4, Pierrette Costes,1, Naima Aron,1, Fabienne Reine,5, Laetitia
Herzog,5, Juan-Carlos Espinosa,2, Vincent Beringue5, & Olivier Andréoletti1,
Affiliations Contributions Corresponding author Journal name: Nature
Communications Volume: 5, Article number: 5821 DOI: doi:10.1038/ncomms6821
Received 07 August 2014 Accepted 10 November 2014 Published 16 December 2014
Article tools Citation Reprints Rights & permissions Article metrics
Abstract
Although Bovine Spongiform Encephalopathy (BSE) is the cause of variant
Creutzfeldt Jakob disease (vCJD) in humans, the zoonotic potential of scrapie
prions remains unknown. Mice genetically engineered to overexpress the human
prion protein (tgHu) have emerged as highly relevant models for gauging the
capacity of prions to transmit to humans. These models can propagate human
prions without any apparent transmission barrier and have been used used to
confirm the zoonotic ability of BSE. Here we show that a panel of sheep scrapie
prions transmit to several tgHu mice models with an efficiency comparable to
that of cattle BSE. The serial transmission of different scrapie isolates in
these mice led to the propagation of prions that are phenotypically identical to
those causing sporadic CJD (sCJD) in humans. These results demonstrate that
scrapie prions have a zoonotic potential and raise new questions about the
possible link between animal and human prions.
Subject terms: Biological sciences• Medical research At a glance
why
do we not want to do TSE transmission studies on chimpanzees $
5.
A positive result from a chimpanzee challenged severly would likely create alarm
in some circles even if the result could not be interpreted for man. I have a
view that all these agents could be transmitted provided a large enough dose by
appropriate routes was given and the animals kept long enough. Until the
mechanisms of the species barrier are more clearly understood it might be best
to retain that hypothesis.
snip...
R.
BRADLEY
FLASHBACK 2001
Suspect symptoms
What if you can catch old-fashioned CJD by eating meat from a sheep
infected with scrapie?
28
Mar 01 Most doctors believe that sCJD is caused by a prion protein deforming by
chance into a killer. But Singeltary thinks otherwise. He is one of a number of
campaigners who say that some sCJD, like the variant CJD related to BSE, is
caused by eating meat from infected animals. Their suspicions have focused on
sheep carrying scrapie, a BSE-like disease that is widespread in flocks across
Europe and North America.
Now
scientists in France have stumbled across new evidence that adds weight to the
campaigners' fears. To their complete surprise, the researchers found that one
strain of scrapie causes the same brain damage in mice as sCJD.
"This means we cannot rule out that at least some sCJD may be caused by
some strains of scrapie," says team member Jean-Philippe Deslys of the French
Atomic Energy Commission's medical research laboratory in Fontenay-aux-Roses,
south-west of Paris. Hans Kretschmar of the University of Göttingen, who
coordinates CJD surveillance in Germany, is so concerned by the findings that he
now wants to trawl back through past sCJD cases to see if any might have been
caused by eating infected mutton or lamb...
2001
Suspect symptoms
What if you can catch old-fashioned CJD by eating meat from a sheep
infected with scrapie?
28
Mar 01
Like lambs to the slaughter
31
March 2001
by
Debora MacKenzie Magazine issue 2284.
FOUR years ago, Terry Singeltary watched his mother die horribly from a
degenerative brain disease. Doctors told him it was Alzheimer's, but Singeltary
was suspicious. The diagnosis didn't fit her violent symptoms, and he demanded
an autopsy. It showed she had died of sporadic Creutzfeldt-Jakob disease.
Most doctors believe that sCJD is caused by a prion protein deforming by
chance into a killer. But Singeltary thinks otherwise. He is one of a number of
campaigners who say that some sCJD, like the variant CJD related to BSE, is
caused by eating meat from infected animals. Their suspicions have focused on
sheep carrying scrapie, a BSE-like disease that is widespread in flocks across
Europe and North America.
Now
scientists in France have stumbled across new evidence that adds weight to the
campaigners' fears. To their complete surprise, the researchers found that one
strain of scrapie causes the same brain damage in mice as sCJD.
"This means we cannot rule out that at least some sCJD may be caused by
some strains of scrapie," says team member Jean-Philippe Deslys of the French
Atomic Energy Commission's medical research laboratory in Fontenay-aux-Roses,
south-west of Paris. Hans Kretschmar of the University of Göttingen, who
coordinates CJD surveillance in Germany, is so concerned by the findings that he
now wants to trawl back through past sCJD cases to see if any might have been
caused by eating infected mutton or lamb.
Scrapie has been around for centuries and until now there has been no
evidence that it poses a risk to human health. But if the French finding means
that scrapie can cause sCJD in people, countries around the world may have
overlooked a CJD crisis to rival that caused by BSE.
Deslys and colleagues were originally studying vCJD, not sCJD. They
injected the brains of macaque monkeys with brain from BSE cattle, and from
French and British vCJD patients. The brain damage and clinical symptoms in the
monkeys were the same for all three. Mice injected with the original sets of
brain tissue or with infected monkey brain also developed the same
symptoms.
As
a control experiment, the team also injected mice with brain tissue from people
and animals with other prion diseases: a French case of sCJD; a French patient
who caught sCJD from human-derived growth hormone; sheep with a French strain of
scrapie; and mice carrying a prion derived from an American scrapie strain. As
expected, they all affected the brain in a different way from BSE and vCJD. But
while the American strain of scrapie caused different damage from sCJD, the
French strain produced exactly the same pathology.
"The main evidence that scrapie does not affect humans has been
epidemiology," says Moira Bruce of the neuropathogenesis unit of the Institute
for Animal Health in Edinburgh, who was a member of the same team as Deslys.
"You see about the same incidence of the disease everywhere, whether or not
there are many sheep, and in countries such as New Zealand with no scrapie." In
the only previous comparisons of sCJD and scrapie in mice, Bruce found they were
dissimilar.
But
there are more than 20 strains of scrapie, and six of sCJD. "You would not
necessarily see a relationship between the two with epidemiology if only some
strains affect only some people," says Deslys. Bruce is cautious about the mouse
results, but agrees they require further investigation. Other trials of scrapie
and sCJD in mice, she says, are in progress.
People can have three different genetic variations of the human prion
protein, and each type of protein can fold up two different ways. Kretschmar has
found that these six combinations correspond to six clinical types of sCJD: each
type of normal prion produces a particular pathology when it spontaneously
deforms to produce sCJD.
But
if these proteins deform because of infection with a disease-causing prion, the
relationship between pathology and prion type should be different, as it is in
vCJD. "If we look at brain samples from sporadic CJD cases and find some that do
not fit the pattern," says Kretschmar, "that could mean they were caused by
infection."
There are 250 deaths per year from sCJD in the US, and a similar
incidence elsewhere. Singeltary and other US activists think that some of these
people died after eating contaminated meat or "nutritional" pills containing
dried animal brain. Governments will have a hard time facing activists like
Singeltary if it turns out that some sCJD isn't as spontaneous as doctors have
insisted.
Deslys's work on macaques also provides further proof that the human
disease vCJD is caused by BSE. And the experiments showed that vCJD is much more
virulent to primates than BSE, even when injected into the bloodstream rather
than the brain. This, says Deslys, means that there is an even bigger risk than
we thought that vCJD can be passed from one patient to another through
contaminated blood transfusions and surgical instruments.
Friday, January 30, 2015
***
Scrapie: a particularly persistent pathogen ***
Tuesday, February 17, 2015
***Could we spot the next BSE?, asks BVA President
Saturday, February 14, 2015
*** Canadian Food Inspection Agency Confirms Bovine Spongiform
Encephalopathy (BSE) in Alberta
Tuesday, February 10, 2015
*** Alberta Canada First case of chronic wasting disease found in farm elk
since 2002
Wednesday, December 31, 2014
NASDA BSE, CWD, SCRAPIE, TSE, PRION, Policy Statements updated with
amendments passed during the NASDA Annual Meeting Updated September 18, 2014
Sunday, December 28, 2014
CHRONIC WASTING DISEASE CWD TSE PRION DISEASE AKA MAD DEER DISIEASE USDA
USAHA INC DECEMBER 28, 2014
Subject: *** Becky Lockhart 46, Utah’s first female House speaker, dies
diagnosed with the extremely rare Creutzfeldt-Jakob disease aka mad cow type
disease
what is CJD ? just ask USDA inc., and the OIE, they are still feeding
the public and the media industry fed junk science that is 30 years old.
why
doesn’t some of you try reading the facts, instead of rubber stamping everything
the USDA inc says.
sporadic CJD has now been linked to BSE aka mad cow disease, Scrapie,
and there is much concern now for CWD and risk factor for humans.
My
sincere condolences to the family and friends of the House Speaker Becky
Lockhart. I am deeply saddened hear this.
with that said, with great respect, I must ask each and every one of you
Politicians that are so deeply saddened to hear of this needless death of the
Honorable House Speaker Becky Lockhart, really, cry me a friggen river. I am
seriously going to ask you all this...I have been diplomatic for about 17 years
and it has got no where. people are still dying. so, are you all stupid or
what??? how many more need to die ??? how much is global trade of beef and other
meat products that are not tested for the TSE prion disease, how much and how
many bodies is this market worth?
Saturday, January 17, 2015
***
Becky Lockhart 46, Utah’s first female House speaker, dies diagnosed with the
extremely rare Creutzfeldt-Jakob disease
Thursday, January 15, 2015
41-year-old Navy Commander with sporadic Creutzfeldt–Jakob disease CJD
TSE Prion: Case Report
***
HUMAN MAD COW DISEASE nvCJD TEXAS CASE NOT LINKED TO EUROPEAN TRAVEL CDC
***
Sunday, November 23, 2014
***
Confirmed Variant Creutzfeldt-Jakob Disease (variant CJD) Case in Texas in June
2014 confirmed as USA case NOT European
the
patient had resided in Kuwait, Russia and Lebanon. The completed investigation
did not support the patient's having had extended travel to European countries,
including the United Kingdom, or travel to Saudi Arabia. The specific overseas
country where this patient’s infection occurred is less clear largely because
the investigation did not definitely link him to a country where other known
vCJD cases likely had been infected.
Sunday, December 14, 2014
ALERT new variant Creutzfeldt Jakob Disease nvCJD or vCJD, sporadic CJD
strains, TSE prion aka Mad Cow Disease United States of America Update December
14, 2014 Report
Sunday, February 08, 2015
FDA
SCIENCE BOARD TO THE FOOD AND DRUG ADMINISTRATION BOVINE HEPARIN BSE CJD TSE
PRION Wednesday, June 4, 2014
Thursday, January 22, 2015
Transmission properties of atypical Creutzfeldt-Jakob disease: a clue to
disease etiology?
Saturday, December 13, 2014
Terry S. Singeltary Sr. Publications TSE prion disease
Diagnosis and Reporting of Creutzfeldt-Jakob Disease
Singeltary, Sr et al. JAMA.2001; 285: 733-734. Vol. 285 No. 6, February
14, 2001 JAMA
snip...
TSS
