atypical, BSE, CWD, Scrapie, Captive Farmed shooting pens (livestock), Wild
Cervids, Rectal Mucosa Biopsy 2012 USAHA Proceedings, and CJD TSE prion
Update
PROCEEDINGS ONE HUNDRED AND SIXTEENTH ANNUAL MEETING of the UNITED STATES
ANIMAL HEALTH ASSOCIATION Sheraton Greensboro Hotel Greensboro, North Carolina
October 18 – 24, 2012
Evaluation and Interpretation of Rectal Mucosa Biopsy Testing for Chronic
Wasting Disease within Four White-Tailed Deer Herds in North America
Bruce V. Thomsen
USDA-APHIS-VS, National Veterinary Services Laboratories (NVSL)
An effective live animal test is needed to assist in the control of chronic
wasting disease (CWD), which has spread through captive and wild herds of
white-tailed deer in both Canada and the United States. Rectal biopsy sample
testing for CWD has shown promising results in previous studies and rectal
biopsy sample testing has also been utilized successfully as a live animal test
to diagnose the closely related disease, scrapie in sheep. This study compared
the test results of postmortem rectal mucosa biopsy samples to those from
conventional postmortem samples of the brainstem at the obex; the medial
retropharyngeal lymph node; and the palatine tonsil in four CWD-infected,
captive white-tailed deer herds. Three of the herds were located in Canada and
one of the herds was from the United States. The effects of age, sex, genotype
at prion protein (PRNP) codon 96, and stage of disease progression were
evaluated as possible factors that might influence test performance. Test
sensitivity for CWD on rectal biopsy samples in whitetailed deer ranged from 63%
to 100% in the four herds within this study. Test performance was influenced by
genotype at PRNP codon 96 and by stage of disease progression. Test sensitivity
was the highest for 96GG deer and lower for 96GS deer. Rectal biopsy test
sensitivity was 100% for deer in the later stages of disease progression, as
evidenced by abundant immunohistochemical staining for PrPCWD in sections of
brainstem. Rectal biopsy test sensitivity was reduced for deer in the earlier
stages of disease. Selective use of this test, in conjunction with conventional
testing postmortem testing, could provide valuable information during disease
investigations of CWD suspect deer herds.
CAPTIVE WILDLIFE AND ALTERNATIVE LIVESTOCK
221
Review and Updates of the USDA-APHIS Veterinary Services (VS)
National Chronic Wasting Disease (CWD) Program
Patrice Klein
USDA-APHIS-VS
CWD Rule Update
CWD Interim Final Rule was published on June 8, 2012, establishing a
national voluntary CWD herd certification program (HCP) and consistent minimum
interstate movement requirements. The rule became effective on August 13, 2012.
Enforcement of the interstate movement regulations is delayed until December 10,
2012 to give States time to apply to APHIS to become an Approved State CWD HCP.
After reviewing the public comments, the APHIS will issue a final rule, and
if needed, incorporate any changes made in response to comments on preemption.
Comments received on other topics will be held for future rulemaking.
The goal of the CWD Program is to assist States, Tribes, and the cervid
industry to prevent and control spread of CWD in farmed and wild cervid
populations through establishment of a national CWD HCP and interstate movement
requirements.
APHIS provides federal oversight of the voluntary national CWD HCP with
program activities conducted by the Approved State CWD HCPs. APHIS will serve in
an advisory capacity to Approved States for epidemiological investigations on
CWD positive findings, development of herd plans, and assist (where possible)
with herd inspections and inventories.
APHIS will continue to fund confirmatory testing on any presumptive
CWD-positive samples from farmed and wild cervids, conducted by the National
Veterinary Services Laboratories (NVSL).
Farmed/captive cervid surveillance testing
Through FY2012, CWD surveillance testing was conducted on approximately
22,585 farmed /captive cervids by the immunohistochemistry (IHC) standard
protocol. This reflects testing that was funded by APHIS through December 2011
and the transition to these laboratory costs paid directly by the cervid owner
beginning in January 2012 as a result of CWD program budget reductions in
FY2012.
Farmed/captive cervid CWD status
To date, 60 farmed/captive cervid herds have been identified in 13 states:
Colorado, Iowa, Kansas, Michigan, Minnesota, Missouri, Montana, Nebraska, New
York, Oklahoma, Pennsylvania, South Dakota and Wisconsin. Forty were elk herds,
19 were whitetail deer (WTD) herds, and one was the red deer herd. At this time,
15 CWD positive herds remain – seven elk herds in Colorado, three elk herds in
Nebraska, three WTD herds in Iowa, one WTD herd in Pennsylvania, and one red
deer herd in Minnesota.
On October 11, 2012, Pennsylvania reported a CWD positive three and
one-half year old female white-tailed deer (WTD) in a farmed cervid herd in
Adams County, Pennsylvania. NVSL conducted the confirmatory CWD testing and this
represents the first report of CWD in PA. The index herd is
REPORT OF THE COMMITTEE
222
under state quarantine, and an epidemiological investigation and trace outs
are in progress to identify epidemiologically-linked premises in Pennsylvania
and other states.
In July, 2012, Iowa reported a CWD positive six year old male WTD in a hunt
facility in Davis County, Iowa that was sourced from a deer breeding farm under
the same ownership in Cerro Gordo County, Iowa. Trace outs identified several
other premises that purchased deer from the index herd. CWD testing of the
traced out animals has begun. To date, one CWD positive doe was identified in
the source herd that had direct contact with the index animal, and four
additional CWD positive deer (including two purchased deer) have been identified
on separately owned premises.
In May 2012, Minnesota reported CWD in a two and one-half year old male red
deer from a breeding farm in Ramsey County, Minnesota. This represents the first
report of CWD in red deer (Cervus elaphus) in the United States. During the
epidemiological investigation, 56 pen mates (cohorts) were tested and CWD was
not detected in any of those animals. No point source of introduction yet has
been determined. The herd remains under state imposed quarantine which is
allowing for some animals to be transported directly to a slaughter facility.
All slaughtered animals have been CWD tested and reported as ‘not detected’.
Wild Cervid surveillance
In FY2011, cooperative agreements were awarded to 46 State wildlife
agencies (approximately $4.2 M) and 34 Native American Tribes (approximately
$340,000). The Native American Fish and Wildlife Society received approximately
$175,000 to support CWD outreach and education activities Cooperative agreement
funds were eliminated in FY2012 due to federal budget reductions.
FY2010 funding supported surveillance in approximately 74,900 wild cervids
in 46 cooperating States. Wild cervid CWD surveillance totals are pending for
FY2011 due to seasonal surveillance activities and completion of final
cooperative agreement reporting to APHIS. To date, approximately 60,890 wild
cervids have been tested in fiscal year 2011.
Budget: Commodity Health Line Structure
In FY2011, APHIS received approximately $15.8 million in appropriated
funding for the CWD Program. In the FY2012 budget, livestock commodities
regulated by USDA were organized into ‘Commodity Health Line’ structures or
groupings. APHIS’ Equine, Cervid and Small Ruminant (ECSR) Health line supports
efforts to protect the health and thereby improve the quality and productivity
of the equine, cervid and small ruminant industries. In FY2012 approximately
$1.925 million of ECSR funding was allocated for CWD program activities to
provide Federal oversight of the national CWD herd certification program (HCP).
The President’s FY2013 budget proposes further funding reductions.
Chronic Wasting Disease (CWD) rule: Clifford reported the Office of
Management and Budget (OMB) wants an update and the rule will not preempt
states.
snip...
RESOLUTION NUMBER: 13 and 23 Combined – APPROVED
SOURCE: Committee on Wildlife Diseases Committee on Captive Wildlife and
Alternative Livestock
SUBJECT MATTER: Funding for Indemnity of Chronic Wasting Disease- Positive
or Exposed Animals BACKGROUND INFORMATION:
The Administrator is authorized to pay for the purchase and destruction of
Chronic Wasting Disease (CWD) positive animals, CWD exposed animals, and CWD
suspect animals (9 CFR 55.2). Subject to available funding, the amount of the
Federal payment for any such animals will be 95 percent of the appraised value
established in accordance with 55.3 of this part, but the Federal payment shall
not exceed $3,000.00 per animal. In the past, the United States Department of
Agriculture, Animal and Plant Health Inspection Service, Veterinary Services has
provided funding to pay for the purchase of farmed cervids that tested positive
for CWD, were exposed to CWD positive animals, or were suspect animals, in order
to mitigate the risk of the spread of CWD to other captive and wild cervids.
Federal funding for this purpose is no longer available and farmed cervidae
producers are no longer indemnified for the destruction of their animals.
Without federal funding for the purchase of destroyed animals, producers will
suffer considerable financial damages.
REPORT OF THE COMMITTEE
392
RESOLUTION:
The United States Animal Health Association urges the United States
Department of Agriculture, Animal and Plant Health Inspection Service,
Veterinary Services to provide funding for a federal program to pay indemnity
for animals euthanized because of infection or exposure to Chronic Wasting
Disease.
*****
RESOLUTION NUMBER: 20– APPROVED
SOURCE: Committee on Captive Wildlife and Alternative Livestock
SUBJECT MATTER: Chronic Wasting Disease Control
BACKGROUND INFORMATION:
It has been stated by the United States Department of Agriculture, Animal
and Plant Health Inspection Service, Veterinary Services that
(1) the goal of the Chronic Wasting Disease (CWD) program in the United
States has now changed from eradication to controlling its spread,
(2) there is no longer federal funding available to pay for CWD testing or
to pay indemnity for CWD infected or exposed animals, and
(3) depopulation of infected herds will no longer be required or expected.
With this major change in objectives, it is critical that we change the way
we implement the CWD program in the United States. We now need a program that
minimizes the risk of spreading CWD in farmed and wild cervidae without putting
farmed cervidae producers out of business if their
NOMINATIONS AND RESOLUTIONS
397
herds become CWD infected or exposed. We need a CWD control program that
includes plans for how to (1) handle infected or exposed herds, (2) clean up
infected herds without depopulation, and (3) provide outlets so producers can
continue to sell velvet antler and live animals to slaughter or specified
terminal facilities.
RESOLUTION:
The United States Animal Health Association urges the United States
Department of Agriculture, Animal and Plant Health Inspection Service,
Veterinary Services and state animal health regulatory officials to develop
protocols for the Chronic Wasting Disease (CWD) control program that mitigate
the risk of the spread of CWD and allow producers with CWD infected or exposed
herds to continue operations under quarantine and which allow (1) addition of
cervidae from CWD certified herds, (2) participation in herd plans such as test
and removal, and (3) movement of velvet antler and live animals to slaughter or
other approved terminal facilities.
*****
RESOLUTION NUMBER: 21 – APPROVED AS AMENDED
SOURCE: Committee on Captive Wildlife and Alternative Livestock
SUBJECT MATTER: Funding for Chronic Wasting Disease Testing
BACKGROUND INFORMATION:
The requirements for Chronic Wasting Disease (CWD) herd certification (9
CFR 55) and for interstate movement of farmed cervidae (9 CFR 81) specify that
all farmed cervidae greater than 12 months of age that die or are slaughtered
must be tested for CWD.
The CWD testing protocol that is recommended for farmed cervidae is the
immunohistochemistry test using formalin fixed samples of brain stem or a
retropharyngeal lymph node. The test on either of these tissues is highly
sensitive and specific for detecting the presence of CWD prion. The test costs
at least $25.00 per slide to perform at United States Department of Agriculture
(USDA) approved laboratories. In the past, USDA, Animal and Plant Health
Inspection Service, Veterinary Services has provided funding to pay for CWD
testing of wild and farmed cervids in the United States. Federal funding for
this purpose is no longer available and farmed cervidae producers in most states
must pay the entire cost for required CWD tests. Without federal funding for CWD
testing, producer compliance with program requirements is likely to decrease.
Without producer support, the program to control the spread of CWD in the United
States may become less effective.
Funding for CWD testing was requested and approved in United States Animal
Health Association 2011 resolution number 14.
REPORT OF THE COMMITTEE
398
RESOLUTION:
The United States Animal Health Association urges Congress to appropriate
federal funding to pay the laboratory costs of testing farmed and wild cervidae
for Chronic Wasting Disease.
*****
RESOLUTION NUMBER: 23 – Combined with 13
SOURCE: Committee on Captive Wildlife and Alternative Livestock
SUBJECT MATTER: Funding for Indemnity of Chronic Wasting Disease Positive
or Exposed Animals
*****
RESOLUTION NUMBER: 24 – APPROVED
SOURCE: Committee on Captive Wildlife and Alternative Livestock S UBJECT
MATTER: Chronic Wasting Disease Program Standards BACKGROUND INFORMATION:
It has been stated by the United States Department of Agriculture (USDA),
Animal and Plant Health Inspection Service (APHIS), Veterinary Services (VS)
that the goal of the Chronic Wasting Disease (CWD) program in the United States
has now changed from eradication to controlling its spread.
The document entitled, "Chronic Wasting Disease Program Standards" was
published by USDA-APHIS-VS in July 2012. It was developed before the shift of
the CWD program from eradication to control and without adequate input from
state wildlife and animal health officials or farmed cervidae producers.
Sections of the document suggest placing restrictions on farmed cervidae
producers that do nothing to further the effort to control the spread of CWD.
The restrictions are not based on current scientific knowledge and could
undermine the success of CWD control programs that have been in place in many
states for more than a decade.
NOMINATIONS AND RESOLUTIONS
399
RESOLUTION:
The United States Animal Health Association urges the United States
Department of Agriculture (USDA), Animal and Plant Health Inspection Service
(APHIS), Veterinary Services (VS) to revise the document entitled, "Chronic
Wasting Disease Program Standards", and establish a Chronic Wasting Disease
(CWD) Program Standards Committee to review and rewrite the document within 90
days so that it more appropriately reflects the needs of producers and
regulatory officials charged with implementation of a program to control, not
eradicate, CWD in the United States.
The United States Animal Health Association suggests that the CWD Program
Standards Committee should be made up of representatives from and appointed by
each of the following organizations:
(1) the Exotic Wildlife Association,
(2) the North American Elk Breeders Association,
(3) the North American Deer Farmers Association,
(4) the Association of Fish and Wildlife Agencies,
(5) the National Assembly of State Animal Health Officials, and
(6) the USDA-APHIS-VS.
*****
snip...
RESOLUTION NUMBER: 26, 9 and 30 Combined – APPROVED
SOURCE: Committee on Scrapie
Committee on Import Export Committee on Sheep and Goats SUBJECT MATTER:
Export of Sheep and Goats BACKGROUND INFORMATION:
Under the National Scrapie Eradication Program the prevalence of scrapie in
the United States flock has decreased significantly over the past 10 years. The
funding for the Scrapie Flock Certification Program (SFCP) has been reduced and
participation by sheep and goat breeders has dramatically decreased. It has
become increasingly difficult to find breeding sheep and goats for export
shipments that meet importing country protocols that rely on SFCP participation.
Additionally, new tools such as genotyping and live-animal testing can be used
to identify sheep that are at low risk for
REPORT OF THE COMMITTEE
400
scrapie. These approaches may provide an appropriate basis for revised
export protocols.
RESOLUTION:
The United States Animal Health Association urges the United States
Department of Agriculture, Animal Health and Plant Inspection Services,
Veterinary Services to expand their negotiating tools for the export of sheep
and goats beyond those that rely on the Scrapie Flock Certification Program
participation alone and to encourage other countries to recognize current
National Scrapie Eradication Program prevalence and surveillance data along with
the use of other tools such as genotyping when appropriate.
*****
snip...
Bovine Spongiform Encephalopathy (BSE) surveillance: Clifford said that if
it was up to USDA, surveillance testing would be scaled back. The US has
requested negligible risk status, but OIE has been informed the US, that we will
not get this status this year.
Activities
Magde Elshafie
USDA-APHIS-VS-NCIE
Bovine Spongiform Encephalopathy (BSE) Comprehensive Rule
The BSE Comprehensive Rule was published March 2012, the comment period
closed in June of 2012. It established BSE-related import provisions which are
more closely aligned with OIE guidelines including country risk status
classifications (Negligible, Controlled, and Undetermined). It also allows
flexibility in the BSE risk classification process allowing Animal and Plant
Health Inspection Service (APHIS) to concur with World Animal Health
Organization (OIE) BSE determinations. However, this will not eliminate
independent APHIS evaluation of any country or region for BSE status. A country
will be considered undetermined risk until such time that APHIS determines it to
be Negligible or Controlled Risk. Recognition will be based on the following
criteria;
1) APHIS concurrence with OIE classification, OR
2) APHIS evaluation, upon request, of countries not classified by the
OIE.
The BSE Comprehensive Rule eliminates the need for formal rulemaking for
each individual country/region. The importation of bovines and bovine products
from BSE minimal-risk regions (Canada) and for boneless beef from Japan would be
removed from the Federal Register and incorporated into the final rule. It will
allow the importation of additional bovine and bovine products into the United
States from all negligible and controlled risk regions using requirements based
on OIE guidelines. • Hides/skins and Gelatin/Collagen from hides/skins
• Deboned meat (excluding methlysulfonylmethane (MSM) from cattle ≤30
months of age provided the animals pass ante- and post-mortem inspection,
specified risk materials (SRM) are removed, and they were not subjected to an
air injected stunning process or pithing
• Protein-free tallow and derivatives made from this tallow • Dicalcium
phosphate with no trace of protein or fat
• Blood/blood by-products derived from cattle not subjected to an air
injected stunning process or pithing, and collected in a manner that avoids
contamination Ruminant meat-and-bone meal (MBM) and greaves from controlled and
undetermined risk countries will remain as prohibited materials. Transmissible
Spongiform Encephalopathies (TSE) Rule OIE Code does not address BSE risk for
ovines/caprines.
Therefore, a separate rule and risk assessment currently under development
that will address import requirements for TSEs and allow importation of sheep
and IMPORT-EXPORT
301
goats, their embryos, and their products/by products from countries
classified as Negligible or Controlled Risk for BSE under certain conditions.
snip...
Friday, August 31, 2012
COMMITTEE ON CAPTIVE WILDLIFE AND ALTERNATIVE LIVESTOCK and CWD 2009-2012 a
review
Tuesday, April 16, 2013
Cervid Industry Unites To Set Direction for CWD Reform and seem to ignore
their ignorance and denial in their role in spreading Chronic Wasting
Disease
Saturday, February 04, 2012
Wisconsin 16 MONTH age limit on testing dead deer Game Farm CWD Testing
Protocol Needs To Be Revised
Thursday, June 13, 2013
WISCONSIN DEER FARMING Chronic Wasting Disease CWD DATCP
Tuesday, June 11, 2013
CWD GONE WILD, More cervid escapees from more shooting pens on the loose in
Pennsylvania
Tuesday, May 28, 2013
Chronic Wasting Disease CWD quarantine Louisiana via CWD index herd
Pennsylvania Update May 28, 2013
6 doe from Pennsylvania CWD index herd still on the loose in Louisiana,
quarantine began on October 18, 2012, still ongoing, Lake Charles premises.
Tuesday, October 23, 2012
PA Captive deer from CWD-positive farm roaming free
> Ag is one of the agencies cooperating in the response plan because it
has responsibility for regulating captive deer and deer farms, of which there
are estimated to be more 23,000 on 1,100 Pennsylvania properties.
Tuesday, November 06, 2012
PA Department of Agriculture investigating possible 2nd case of chronic
wasting disease
Thursday, November 01, 2012
PA GAME COMMISSION TO HOLD PUBLIC MEETING TO DISCUSS CWD Release #128-12
Friday, October 26, 2012
CHRONIC WASTING DISEASE CWD PENNSYLVANIA GAME FARMS, URINE ATTRACTANT
PRODUCTS, BAITING, AND MINERAL LICKS
Tuesday, October 23, 2012
PA Captive deer from CWD-positive farm roaming free
Monday, October 15, 2012
PENNSYLVANIA GAME COMMISSION AND AGRICULTURE DEPARTMENT TO HOLD PUBLIC
MEETING TO DISCUSS CWD MONITORING EFFORTS FOR IMMEDIATE RELEASE: October 15,
2012 Release #124-12
Thursday, October 11, 2012
Pennsylvania Confirms First Case CWD Adams County Captive Deer Tests
Positive
Pennsylvania CWD number of deer exposed and farms there from much greater
than first thought
Published: Wednesday, October 17, 2012, 10:44 PM Updated: Wednesday,
October 17, 2012, 11:33 PM
snip...see full history of this with references here ;
Wednesday, November 14, 2012
PENNSYLVANIA 2012 THE GREAT ESCAPE OF CWD INVESTIGATION MOVES INTO
LOUISIANA and INDIANA
Saturday, June 08, 2013
The battle against Chronic Wasting Disease continues in Southeast
Iowa
Sunday, June 09, 2013
Missouri House forms 13-member Interim Committee on the Cause and Spread of
Chronic Wasting Disease CWD
Monday, June 11, 2012
OHIO Captive deer escapees and non-reporting
Friday, September 28, 2012
Stray elk renews concerns about deer farm security Minnesota
Tuesday, July 10, 2012
Dr. James C. Kroll Texas deer czar final report on Wisconsin
Friday, June 01, 2012
*** TEXAS DEER CZAR TO WISCONSIN ASK TO EXPLAIN COMMENTS
snip...
yep, while the Texas deer czar dr. dough was off to Wisconsin pushing the
privately owned shooting pen industry (livestock cervids industry), Texas fell
to CWD, and Texas just reported 4 more CWD postives. ...
for your information...
According to Wisconsin’s White-Tailed Deer Trustee Dr. James Kroll, people
who call for more public hunting opportunities are “pining for socialism.” He
further states, “(Public) Game management is the last bastion of communism.”
“Game Management,” says James Kroll, driving to his high-fenced,
two-hundred-acre spread near Nacogdoches, “is the last bastion of communism.”
Kroll, also known as Dr. Deer, is the director of the Forestry Resources
Institute of Texas at Stephen F. Austin State University, and the “management”
he is referring to is the sort practiced by the State of Texas. The 55-year-old
Kroll is the leading light in the field of private deer management as a means to
add value to the land. His belief is so absolute that some detractors refer to
him as Dr. Dough, implying that his eye is on the bottom line more than on the
natural world.
Kroll, who has been the foremost proponent of deer ranching in Texas for
more than thirty years, doesn’t mind the controversy and certainly doesn’t fade
in the heat. People who call for more public lands are “cocktail
conservationists,” he says, who are really pining for socialism. He calls
national parks “wildlife ghettos” and flatly accuses the government of gross
mismanagement. He argues that his relatively tiny acreage, marked by eight-foot
fences and posted signs warning off would-be poachers, is a better model for
keeping what’s natural natural while making money off the land.
snip...
Thursday, March 29, 2012
TEXAS DEER CZAR SAYS WISCONSIN DNR NOT DOING ENOUGH ABOUT CWD LIKE POT
CALLING KETTLE BLACK
Tuesday, July 10, 2012
Chronic Wasting Disease Detected in Far West Texas
Monday, February 11, 2013
TEXAS CHRONIC WASTING DISEASE CWD Four New Positives Found in Trans Pecos
Tuesday, December 18, 2012
A Growing Threat How deer breeding could put public trust wildlife at risk
Sunday, January 22, 2012
Chronic Wasting Disease CWD cervids interspecies transmission
Friday, November 09, 2012
*** Chronic Wasting Disease CWD in cervidae and transmission to other
species
Friday, November 09, 2012
*** Chronic Wasting Disease CWD in cervidae and transmission to other
species
Sunday, November 11, 2012
*** Susceptibilities of Nonhuman Primates to Chronic Wasting Disease
November 2012
The chances of a person or domestic animal contracting CWD are “extremely
remote,” Richards said. The possibility can’t be ruled out, however. “One could
look at it like a game of chance,” he explained. “The odds (of infection)
increase over time because of repeated exposure. That’s one of the downsides of
having CWD in free-ranging herds: We’ve got this infectious agent out there that
we can never say never to in terms of (infecting) people and domestic
livestock.”
P35
ADAPTATION OF CHRONIC WASTING DISEASE (CWD) INTO HAMSTERS, EVIDENCE OF A
WISCONSIN STRAIN OF CWD
Chad Johnson1, Judd Aiken2,3,4 and Debbie McKenzie4,5 1 Department of
Comparative Biosciences, University of Wisconsin, Madison WI, USA 53706 2
Department of Agriculture, Food and Nutritional Sciences, 3 Alberta Veterinary
Research Institute, 4.Center for Prions and Protein Folding Diseases, 5
Department of Biological Sciences, University of Alberta, Edmonton AB, Canada
T6G 2P5
The identification and characterization of prion strains is increasingly
important for the diagnosis and biological definition of these infectious
pathogens. Although well-established in scrapie and, more recently, in BSE,
comparatively little is known about the possibility of prion strains in chronic
wasting disease (CWD), a disease affecting free ranging and captive cervids,
primarily in North America. We have identified prion protein variants in the
white-tailed deer population and demonstrated that Prnp genotype affects the
susceptibility/disease progression of white-tailed deer to CWD agent. The
existence of cervid prion protein variants raises the likelihood of distinct CWD
strains. Small rodent models are a useful means of identifying prion strains. We
intracerebrally inoculated hamsters with brain homogenates and phosphotungstate
concentrated preparations from CWD positive hunter-harvested (Wisconsin CWD
endemic area) and experimentally infected deer of known Prnp genotypes. These
transmission studies resulted in clinical presentation in primary passage of
concentrated CWD prions. Subclinical infection was established with the other
primary passages based on the detection of PrPCWD in the brains of hamsters and
the successful disease transmission upon second passage. Second and third
passage data, when compared to transmission studies using different CWD inocula
(Raymond et al., 2007) indicate that the CWD agent present in the Wisconsin
white-tailed deer population is different than the strain(s) present in elk,
mule-deer and white-tailed deer from the western United States endemic region.
PPo3-7:
Prion Transmission from Cervids to Humans is Strain-dependent
Qingzhong Kong, Shenghai Huang,*Fusong Chen, Michael Payne, Pierluigi
Gambetti and Liuting Qing Department of Pathology; Case western Reserve
University; Cleveland, OH USA *Current address: Nursing Informatics; Memorial
Sloan-Kettering Cancer Center; New York, NY USA
Key words: CWD, strain, human transmission
Chronic wasting disease (CWD) is a widespread prion disease in cervids
(deer and elk) in North America where significant human exposure to CWD is
likely and zoonotic transmission of CWD is a concern. Current evidence indicates
a strong barrier for transmission of the classical CWD strain to humans with the
PrP-129MM genotype. A few recent reports suggest the presence of two or more CWD
strains. What remain unknown is whether individuals with the PrP-129VV/MV
genotypes are also resistant to the classical CWD strain and whether humans are
resistant to all natural or adapted cervid prion strains. Here we report that a
human prion strain that had adopted the cervid prion protein (PrP) sequence
through passage in cervidized transgenic mice efficiently infected transgenic
mice expressing human PrP, indicating that the species barrier from cervid to
humans is prion strain-dependent and humans can be vulnerable to novel cervid
prion strains. Preliminary results on CWD transmission in transgenic mice
expressing human PrP-129V will also be discussed.
Acknowledgement Supported by NINDS NS052319 and NIA AG14359.
PPo2-27:
Generation of a Novel form of Human PrPSc by Inter-species Transmission of
Cervid Prions
Marcelo A. Barria,1 Glenn C. Telling,2 Pierluigi Gambetti,3 James A.
Mastrianni4 and Claudio Soto1 1Mitchell Center for Alzheimer's disease and
related Brain disorders; Dept of Neurology; University of Texas Houston Medical
School; Houston, TX USA; 2Dept of Microbiology, Immunology & Molecular
Genetics and Neurology; Sanders Brown Center on Aging; University of Kentucky
Medical Center; Lexington, KY USA; 3Institute of Pathology; Case western Reserve
University; Cleveland, OH USA; 4Dept of Neurology; University of Chicago;
Chicago, IL USA
Prion diseases are infectious neurodegenerative disorders affecting humans
and animals that result from the conversion of normal prion protein (PrPC) into
the misfolded and infectious prion (PrPSc). Chronic wasting disease (CWD) of
cervids is a prion disorder of increasing prevalence within the United States
that affects a large population of wild and captive deer and elk. CWD is highly
contagious and its origin, mechanism of transmission and exact prevalence are
currently unclear. The risk of transmission of CWD to humans is unknown.
Defining that risk is of utmost importance, considering that people have been
infected by animal prions, resulting in new fatal diseases. To study the
possibility that human PrPC can be converted into the infectious form by CWD
PrPSc we performed experiments using the Protein Misfolding Cyclic Amplification
(PMCA) technique, which mimic in vitro the process of prion replication. Our
results show that cervid PrPSc can induce the pathological conversion of human
PrPC, but only after the CWD prion strain has been stabilized by successive
passages in vitro or in vivo. Interestingly, this newly generated human PrPSc
exhibits a distinct biochemical pattern that differs from any of the currently
known forms of human PrPSc, indicating that it corresponds to a novel human
prion strain. Our findings suggest that CWD prions have the capability to infect
humans, and that this ability depends on CWD strain adaptation, implying that
the risk for human health progressively increases with the spread of CWD among
cervids.
PPo2-7:
Biochemical and Biophysical Characterization of Different CWD
Isolates
Martin L. Daus and Michael Beekes Robert Koch Institute; Berlin,
Germany
Key words: CWD, strains, FT-IR, AFM
Chronic wasting disease (CWD) is one of three naturally occurring forms of
prion disease. The other two are Creutzfeldt-Jakob disease in humans and scrapie
in sheep. CWD is contagious and affects captive as well as free ranging cervids.
As long as there is no definite answer of whether CWD can breach the species
barrier to humans precautionary measures especially for the protection of
consumers need to be considered. In principle, different strains of CWD may be
associated with different risks of transmission to humans. Sophisticated strain
differentiation as accomplished for other prion diseases has not yet been
established for CWD. However, several different findings indicate that there
exists more than one strain of CWD agent in cervids. We have analysed a set of
CWD isolates from white-tailed deer and could detect at least two biochemically
different forms of disease-associated prion protein PrPTSE. Limited proteolysis
with different concentrations of proteinase K and/or after exposure of PrPTSE to
different pH-values or concentrations of Guanidinium hydrochloride resulted in
distinct isolate-specific digestion patterns. Our CWD isolates were also
examined in protein misfolding cyclic amplification studies. This showed
different conversion activities for those isolates that had displayed
significantly different sensitivities to limited proteolysis by PK in the
biochemical experiments described above. We further applied Fourier transform
infrared spectroscopy in combination with atomic force microscopy. This
confirmed structural differences in the PrPTSE of at least two disinct CWD
isolates. The data presented here substantiate and expand previous reports on
the existence of different CWD strains.
2012
Envt.06:
Zoonotic Potential of CWD: Experimental Transmissions to Non-Human Primates
Emmanuel Comoy,1,† Valérie Durand,1 Evelyne Correia,1 Aru Balachandran,2
Jürgen Richt,3 Vincent Beringue,4 Juan-Maria Torres,5 Paul Brown,1 Bob Hills6
and Jean-Philippe Deslys1
1Atomic Energy Commission; Fontenay-aux-Roses, France; 2Canadian Food
Inspection Agency; Ottawa, ON Canada; 3Kansas State University; Manhattan, KS
USA; 4INRA; Jouy-en-Josas, France; 5INIA; Madrid, Spain; 6Health Canada; Ottawa,
ON Canada
†Presenting author; Email: emmanuel.comoy@cea.fr
The constant increase of chronic wasting disease (CWD) incidence in North
America raises a question about their zoonotic potential. A recent publication
showed their transmissibility to new-world monkeys, but no transmission to
old-world monkeys, which are phylogenetically closer to humans, has so far been
reported. Moreover, several studies have failed to transmit CWD to transgenic
mice overexpressing human PrP. Bovine spongiform encephalopathy (BSE) is the
only animal prion disease for which a zoonotic potential has been proven. We
described the transmission of the atypical BSE-L strain of BSE to cynomolgus
monkeys, suggesting a weak cattle-to-primate species barrier. We observed the
same phenomenon with a cattleadapted strain of TME (Transmissible Mink
Encephalopathy). Since cattle experimentally exposed to CWD strains have also
developed spongiform encephalopathies, we inoculated brain tissue from
CWD-infected cattle to three cynomolgus macaques as well as to transgenic mice
overexpressing bovine or human PrP. Since CWD prion strains are highly
lymphotropic, suggesting an adaptation of these agents after peripheral
exposure, a parallel set of four monkeys was inoculated with CWD-infected cervid
brains using the oral route. Nearly four years post-exposure, monkeys exposed to
CWD-related prion strains remain asymptomatic. In contrast, bovinized and
humanized transgenic mice showed signs of infection, suggesting that CWD-related
prion strains may be capable of crossing the cattle-to-primate species barrier.
Comparisons with transmission results and incubation periods obtained after
exposure to other cattle prion strains (c-BSE, BSE-L, BSE-H and cattle-adapted
TME) will also be presented, in order to evaluate the respective risks of each
strain.
Envt.07:
Pathological Prion Protein (PrPTSE) in Skeletal Muscles of Farmed and Free
Ranging White-Tailed Deer Infected with Chronic Wasting Disease
Martin L. Daus,1,† Johanna Breyer,2 Katjs Wagenfuehr,1 Wiebke Wemheuer,2
Achim Thomzig,1 Walter Schulz-Schaeffer2 and Michael Beekes1 1Robert Koch
Institut; P24 TSE; Berlin, Germany; 2Department of Neuropathology, Prion and
Dementia Research Unit, University Medical Center Göttingen; Göttingen, Germany
†Presenting author; Email: dausm@rki.de
Chronic wasting disease (CWD) is a contagious, rapidly spreading
transmissible spongiform encephalopathy (TSE) occurring in cervids in North
America. Despite efficient horizontal transmission of CWD among cervids natural
transmission of the disease to other species has not yet been observed. Here, we
report a direct biochemical demonstration of pathological prion protein PrPTSE
and of PrPTSE-associated seeding activity in skeletal muscles of CWD-infected
cervids. The presence of PrPTSE was detected by Western- and postfixed frozen
tissue blotting, while the seeding activity of PrPTSE was revealed by protein
misfolding cyclic amplification (PMCA). The concentration of PrPTSE in skeletal
muscles of CWD-infected WTD was estimated to be approximately 2000- to
10000-fold lower than in brain tissue. Tissue-blot-analyses revealed that PrPTSE
was located in muscle- associated nerve fascicles but not, in detectable
amounts, in myocytes. The presence and seeding activity of PrPTSE in skeletal
muscle from CWD-infected cervids suggests prevention of such tissue in the human
diet as a precautionary measure for food safety, pending on further
clarification of whether CWD may be transmissible to humans.
Friday, December 14, 2012
Susceptibility Chronic Wasting Disease (CWD) in wild cervids to Humans 2005
- December 14, 2012
Saturday, March 09, 2013
Chronic Wasting Disease in Bank Voles: Characterisation of the Shortest
Incubation Time Model for Prion Diseases
pens, pens, PENS ???
*** Spraker suggested an interesting explanation for the occurrence of CWD.
The deer pens at the Foot Hills Campus were built some 30-40 years ago by a Dr.
Bob Davis. At or abut that time, allegedly, some scrapie work was conducted at
this site. When deer were introduced to the pens they occupied ground that had
previously been occupied by sheep.
now, decades later ;
2012
PO-039: A comparison of scrapie and chronic wasting disease in white-tailed
deer
Justin Greenlee, Jodi Smith, Eric Nicholson US Dept. Agriculture;
Agricultural Research Service, National Animal Disease Center; Ames, IA USA
Interspecies transmission studies afford the opportunity to better
understand the potential host range and origins of prion diseases. The purpose
of these experiments was to determine susceptibility of white-tailed deer (WTD)
to scrapie and to compare the resultant clinical signs, lesions, and molecular
profiles of PrPSc to those of chronic wasting disease (CWD). We inoculated WTD
intracranially (IC; n = 5) and by a natural route of exposure (concurrent oral
and intranasal (IN); n = 5) with a US scrapie isolate. All deer were inoculated
with a 10% (wt/vol) brain homogenate from sheep with scrapie (1ml IC, 1 ml IN,
30 ml oral). All deer inoculated by the intracranial route had evidence of PrPSc
accumulation. PrPSc was detected in lymphoid tissues as early as 7
months-post-inoculation (PI) and a single deer that was necropsied at 15.6
months had widespread distribution of PrPSc highlighting that PrPSc is widely
distributed in the CNS and lymphoid tissues prior to the onset of clinical
signs. IC inoculated deer necropsied after 20 months PI (3/5) had clinical
signs, spongiform encephalopathy, and widespread distribution of PrPSc in neural
and lymphoid tissues. The results of this study suggest that there are many
similarities in the manifestation of CWD and scrapie in WTD after IC inoculation
including early and widespread presence of PrPSc in lymphoid tissues, clinical
signs of depression and weight loss progressing to wasting, and an incubation
time of 21-23 months. Moreover, western blots (WB) done on brain material from
the obex region have a molecular profile similar to CWD and distinct from
tissues of the cerebrum or the scrapie inoculum. However, results of microscopic
and IHC examination indicate that there are differences between the lesions
expected in CWD and those that occur in deer with scrapie: amyloid plaques were
not noted in any sections of brain examined from these deer and the pattern of
immunoreactivity by IHC was diffuse rather than plaque-like. After a natural
route of exposure, 100% of WTD were susceptible to scrapie. Deer developed
clinical signs of wasting and mental depression and were necropsied from 28 to
33 months PI. Tissues from these deer were positive for PrPSc by IHC and WB.
Similar to IC inoculated deer, samples from these deer exhibited two different
molecular profiles: samples from obex resembled CWD whereas those from cerebrum
were similar to the original scrapie inoculum. On further examination by WB
using a panel of antibodies, the tissues from deer with scrapie exhibit
properties differing from tissues either from sheep with scrapie or WTD with
CWD. Samples from WTD with CWD or sheep with scrapie are strongly immunoreactive
when probed with mAb P4, however, samples from WTD with scrapie are only weakly
immunoreactive. In contrast, when probed with mAb’s 6H4 or SAF 84, samples from
sheep with scrapie and WTD with CWD are weakly immunoreactive and samples from
WTD with scrapie are strongly positive. This work demonstrates that WTD are
highly susceptible to sheep scrapie, but on first passage, scrapie in WTD is
differentiable from CWD.
2011
*** After a natural route of exposure, 100% of white-tailed deer were
susceptible to scrapie.
Scrapie in Deer: Comparisons and Contrasts to Chronic Wasting Disease (CWD)
Justin J. Greenlee of the Virus and Prion Diseases Research Unit, National
Animal Disease Center, ARS, USDA, Ames, IA provided a presentation on scrapie
and CWD in inoculated deer. Interspecies transmission studies afford the
opportunity to better understand the potential host range and origins of prion
diseases. We inoculated white-tailed deer intracranially (IC) and by a natural
route of exposure (concurrent oral and intranasal inoculation) with a US scrapie
isolate. All deer inoculated by the intracranial route had evidence of PrPSc
accumulation and those necropsied after 20 months post-inoculation (PI) (3/5)
had clinical signs, spongiform encephalopathy, and widespread distribution of
PrPSc in neural and lymphoid tissues. A single deer that was necropsied at 15.6
months PI did not have clinical signs, but had widespread distribution of PrPSc.
This highlights the facts that 1) prior to the onset of clinical signs PrPSc is
widely distributed in the CNS and lymphoid tissues and 2) currently used
diagnostic methods are sufficient to detect PrPSc prior to the onset of clinical
signs. The results of this study suggest that there are many similarities in the
manifestation of CWD and scrapie in white-tailed deer after IC inoculation
including early and widespread presence of PrPSc in lymphoid tissues, clinical
signs of depression and weight loss progressing to wasting, and an incubation
time of 21-23 months. Moreover, western blots (WB) done on brain material from
the obex region have a molecular profile consistent with CWD and distinct from
tissues of the cerebrum or the scrapie inoculum. However, results of microscopic
and IHC examination indicate that there are differences between the lesions
expected in CWD and those that occur in deer with scrapie: amyloid plaques were
not noted in any sections of brain examined from these deer and the pattern of
immunoreactivity by IHC was diffuse rather than plaque-like. After a natural
route of exposure, 100% of white-tailed deer were susceptible to scrapie. Deer
developed clinical signs of wasting and mental depression and were necropsied
from 28 to 33 months PI. Tissues from these deer were positive for scrapie by
IHC and WB. Tissues with PrPSc immunoreactivity included brain, tonsil,
retropharyngeal and mesenteric lymph nodes, hemal node, Peyer’s patches, and
spleen. While two WB patterns have been detected in brain regions of deer
inoculated by the natural route, unlike the IC inoculated deer, the pattern
similar to the scrapie inoculum predominates.
Committee Business:
The Committee discussed and approved three resolutions regarding CWD. They
can be found in the report of the Reswolutions Committee. Essentially the
resolutions urged USDA-APHIS-VS to:
Continue to provide funding for CWD testing of captive cervids
Finalize and publish the national CWD rule for Herd Certification and
Interstate Movement
Evaluate live animal test, including rectal mucosal biopsy, for CWD in
cervids
2011 Annual Report
Research Project: TRANSMISSION, DIFFERENTIATION, AND PATHOBIOLOGY OF
TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES Location: Virus and Prion Research
Unit
2011 Annual Report
In Objective 1, Assess cross-species transmissibility of transmissible
spongiform encephalopathies (TSEs) in livestock and wildlife, numerous
experiments assessing the susceptibility of various TSEs in different host
species were conducted. Most notable is deer inoculated with scrapie, which
exhibits similarities to chronic wasting disease (CWD) in deer suggestive of
sheep scrapie as an origin of CWD.
snip...
4.Accomplishments 1. Deer inoculated with domestic isolates of sheep
scrapie. Scrapie-affected deer exhibit 2 different patterns of disease
associated prion protein. In some regions of the brain the pattern is much like
that observed for scrapie, while in others it is more like chronic wasting
disease (CWD), the transmissible spongiform encephalopathy typically associated
with deer. This work conducted by ARS scientists at the National Animal Disease
Center, Ames, IA suggests that an interspecies transmission of sheep scrapie to
deer may have been the origin of CWD. This is important for husbandry practices
with both captive deer, elk and sheep for farmers and ranchers attempting to
keep their herds and flocks free of CWD and scrapie.
White-tailed Deer are Susceptible to Scrapie by Natural Route of Infection
Jodi D. Smith, Justin J. Greenlee, and Robert A. Kunkle; Virus and Prion
Research Unit, National Animal Disease Center, USDA-ARS
Interspecies transmission studies afford the opportunity to better
understand the potential host range and origins of prion diseases. Previous
experiments demonstrated that white-tailed deer are susceptible to sheep-derived
scrapie by intracranial inoculation. The purpose of this study was to determine
susceptibility of white-tailed deer to scrapie after a natural route of
exposure. Deer (n=5) were inoculated by concurrent oral (30 ml) and intranasal
(1 ml) instillation of a 10% (wt/vol) brain homogenate derived from a sheep
clinically affected with scrapie. Non-inoculated deer were maintained as
negative controls. All deer were observed daily for clinical signs. Deer were
euthanized and necropsied when neurologic disease was evident, and tissues were
examined for abnormal prion protein (PrPSc) by immunohistochemistry (IHC) and
western blot (WB). One animal was euthanized 15 months post-inoculation (MPI)
due to an injury. At that time, examination of obex and lymphoid tissues by IHC
was positive, but WB of obex and colliculus were negative. Remaining deer
developed clinical signs of wasting and mental depression and were necropsied
from 28 to 33 MPI. Tissues from these deer were positive for scrapie by IHC and
WB. Tissues with PrPSc immunoreactivity included brain, tonsil, retropharyngeal
and mesenteric lymph nodes, hemal node, Peyer’s patches, and spleen. This work
demonstrates for the first time that white-tailed deer are susceptible to sheep
scrapie by potential natural routes of inoculation. In-depth analysis of tissues
will be done to determine similarities between scrapie in deer after
intracranial and oral/intranasal inoculation and chronic wasting disease
resulting from similar routes of inoculation.
see full text ;
how many states have $465,000., and can quarantine and purchase there from,
each cwd said infected farm, but how many states can afford this for all the cwd
infected cervid game ranch type farms ???
howmany (?) game farms in a state X $465,000., do all these game farms have
insurance to pay for this risk of infected the wild cervid herds, in each state
???
how many game farms, are too many game farms ?
when you have states handing out shooting pen permits like candy on
halloween, just to advance their coffers, then other states wanting to do the
same thing, with most all of them ignoring the science on shooting pens and cwd,
what do you expect is going to happen.
when is enough, enough ?
Tuesday, December 20, 2011
CHRONIC WASTING DISEASE CWD WISCONSIN Almond Deer (Buckhorn Flats) Farm
Update DECEMBER 2011
The CWD infection rate was nearly 80%, the highest ever in a North American
captive herd.
RECOMMENDATION: That the Board approve the purchase of 80 acres of land for
$465,000 for the Statewide Wildlife Habitat Program in Portage County and
approve the restrictions on public use of the site.
Form 1100-001
(R 2/11)
NATURAL RESOURCES BOARD AGENDA ITEM
SUBJECT: Information Item: Almond Deer Farm Update
FOR: DECEMBER 2011 BOARD MEETING
TUESDAY
TO BE PRESENTED BY TITLE: Tami Ryan, Wildlife Health Section Chief
SUMMARY:
SEE MORE USAHA REPORTS HERE, 2012 NOT PUBLISHED YET...TSS
Friday, December 14, 2012
DEFRA U.K. What is the risk of Chronic Wasting Disease CWD being introduced
into Great Britain? A Qualitative Risk Assessment October 2012
snip...
In the USA, under the Food and Drug Administration’s BSE Feed Regulation
(21 CFR 589.2000) most material (exceptions include milk, tallow, and gelatin)
from deer and elk is prohibited for use in feed for ruminant animals. With
regards to feed for non-ruminant animals, under FDA law, CWD positive deer may
not be used for any animal feed or feed ingredients. For elk and deer considered
at high risk for CWD, the FDA recommends that these animals do not enter the
animal feed system. However, this recommendation is guidance and not a
requirement by law.
Animals considered at high risk for CWD include:
1) animals from areas declared to be endemic for CWD and/or to be CWD
eradication zones and
2) deer and elk that at some time during the 60-month period prior to
slaughter were in a captive herd that contained a CWD-positive animal.
Therefore, in the USA, materials from cervids other than CWD positive
animals may be used in animal feed and feed ingredients for non-ruminants.
The amount of animal PAP that is of deer and/or elk origin imported from
the USA to GB can not be determined, however, as it is not specified in TRACES.
It may constitute a small percentage of the 8412 kilos of non-fish origin
processed animal proteins that were imported from US into GB in 2011.
Overall, therefore, it is considered there is a __greater than negligible
risk___ that (nonruminant) animal feed and pet food containing deer and/or elk
protein is imported into GB.
There is uncertainty associated with this estimate given the lack of data
on the amount of deer and/or elk protein possibly being imported in these
products.
snip...
36% in 2007 (Almberg et al., 2011). In such areas, population declines of
deer of up to 30 to 50% have been observed (Almberg et al., 2011). In areas of
Colorado, the prevalence can be as high as 30% (EFSA, 2011). The clinical signs
of CWD in affected adults are weight loss and behavioural changes that can span
weeks or months (Williams, 2005). In addition, signs might include excessive
salivation, behavioural alterations including a fixed stare and changes in
interaction with other animals in the herd, and an altered stance (Williams,
2005). These signs are indistinguishable from cervids experimentally infected
with bovine spongiform encephalopathy (BSE). Given this, if CWD was to be
introduced into countries with BSE such as GB, for example, infected deer
populations would need to be tested to differentiate if they were infected with
CWD or BSE to minimise the risk of BSE entering the human food-chain via
affected venison.
snip...
The rate of transmission of CWD has been reported to be as high as 30% and
can approach 100% among captive animals in endemic areas (Safar et al., 2008).
snip...
In summary, in endemic areas, there is a medium probability that the soil
and surrounding environment is contaminated with CWD prions and in a
bioavailable form. In rural areas where CWD has not been reported and deer are
present, there is a greater than negligible risk the soil is contaminated with
CWD prion.
snip...
In summary, given the volume of tourists, hunters and servicemen moving
between GB and North America, the probability of at least one person travelling
to/from a CWD affected area and, in doing so, contaminating their clothing,
footwear and/or equipment prior to arriving in GB is greater than negligible.
For deer hunters, specifically, the risk is likely to be greater given the
increased contact with deer and their environment. However, there is significant
uncertainty associated with these estimates.
snip...
Therefore, it is considered that farmed and park deer may have a higher
probability of exposure to CWD transferred to the environment than wild deer
given the restricted habitat range and higher frequency of contact with tourists
and returning GB residents.
snip...
SNIP...SEE ;
Friday, December 14, 2012
DEFRA U.K. What is the risk of Chronic Wasting Disease CWD being introduced
into Great Britain? A Qualitative Risk Assessment October 2012
how many states have $465,000., and can quarantine and purchase there from,
each cwd said infected farm, but how many states can afford this for all the cwd
infected cervid game ranch type farms ???
Tuesday, December 20, 2011
CHRONIC WASTING DISEASE CWD WISCONSIN Almond Deer (Buckhorn Flats) Farm
Update DECEMBER 2011
The CWD infection rate was nearly 80%, the highest ever in a North American
captive herd.
RECOMMENDATION: That the Board approve the purchase of 80 acres of land for
$465,000 for the Statewide Wildlife Habitat Program in Portage County and
approve the restrictions on public use of the site.
SUMMARY:
snip...
C. The DNR will begin timber removal from outside the fence this winter.
Timber removal from inside the fence has begun with hazardous trees removed. The
construction of a second fence 10 – 12 feet outside the present fence will begin
in the spring. This will add an additional level of security for keeping wild
deer from entering the farm and maintain the integrity of the perimeter (see
attachment).
D. The DNR plans to use the Almond Farm as a CWD research facility. Because
the question of how long a contaminated site is a risk to deer is of national
and international interest, there may be opportunities for research and funding
at this facility. One way to potentially assess whether there is a risk to deer
from the Almond Farm is to conduct bioassays focusing on prions persisting in
soil and what role environmental contamination plays in disease transmission. A
proposal is pending from the University of Wisconsin – Stevens Point that
concerns prion degradation via composting. The group is seeking additional
funding from the University of Wisconsin – Madison and representatives in
Canada. USGS is also contemplating a proposal contingent on funding from their
pending federal budget. Any proposed research that includes bringing captive
cervids onto the property will be thoroughly reviewed by the CWD Research
Committee consisting of the Wildlife Health Team, the Wildlife Policy Team, and
Department administration as well as external CWD experts prior to permission
being granted to ensure that the health of the wild deer herd will not be
endangered. The double fencing described above will be critical to minimize the
risk of ingress of free-ranging and egress of any experimental captive cervids.
E. The house is rented and currently occupied by a Northeast district wildlife
employee. The Lessee agrees to perform weekly fence inspections to insure that
the fence integrity has not been compromised. The Lessee also pays for all
utilities, and will provide lawn care, snow removal, gutter cleaning, and other
miscellaneous maintenance as needed. In exchange for these services the monthly
rental fee has been waived. It is agreed that the Lessor and the Lessee shall
review said waiver of the monthly rental charge at the end of every twelve
months that this lease is in effect (see attachment).
snip...
Despite the five year premise plan and site decontamination, The WI DNR has
concerns over the bioavailability of infectious prions at this site to wild
white-tail deer should these fences be removed. Current research indicates that
prions can persist in soil for a minimum of 3 years. However, Georgsson et al.
(2006) concluded that prions that produced scrapie disease in sheep remained
bioavailable and infectious for at least 16 years in natural Icelandic
environments, most likely in contaminated soil. Additionally, the authors
reported that from 1978-2004, scrapie recurred on 33 sheep farms, of which 9
recurrences occurred 14-21 years after initial culling and subsequent restocking
efforts; these findings further emphasize the effect of environmental
contamination on sustaining TSE infectivity and that long-term persistence of
prions in soils may be substantially greater than previously thought. Evidence
of environmental transmission also was documented in a Colorado research
facility where mule deer became infected with CWD in two of three paddocks where
infected deer carcasses had decomposed on site 1.8 years earlier, and in one of
three paddocks where infected deer had last resided 2.2 years earlier (Miller et
al. 2004).
snip...
Environmental contamination has been identified as a possible cause of
recurrence of CWD-infection on elk farms in Canada, when elk were reintroduced
one year after depopulation, clean up and disinfection. To date, 8 CWD infected
farms remain under CFIA (government of Canada) quarantine indefinitely and will
not be allowed to repopulate with cervids until there is additional research on
detection of prions in soils and better understanding of the duration of
persistence of disease-causing prion post depopulation of CWD-infected cervid
farms (Douglas, CFIA, pers. comm.).
Furthermore, the likely transmission of CWD via soil is corroborated by
recent studies showing long-term persistence of prions in soil, that prion binds
to soil components with high affinity and is not easily removed by water, and
that oral prion disease transmission may be enhanced when bound to soil (Johnson
et al. 2006, Schramm et al. 2006, Johnson et al. 2007). These findings suggest
that soil may harbor more TSE infectivity and contribute more significantly to
TSE transmission than previously recognized. These studies highlight the
concerns about the risk of transmission via environmental contamination beyond
five years and that efforts should be made to prevent freeranging deer from
coming into contact with these contaminated facilities.
SNIP...
CHAPTER TWO
OBJECTIVE FOR PROPERTY
Maintain the Perimeter Deer Fence
Wednesday, November 16, 2011
Wisconsin Creutzfeldt Jakob Disease, CWD, TSE, PRION REPORTING 2011
Monday, January 16, 2012
9 GAME FARMS IN WISCONSIN TEST POSITIVE FOR CWD
Tuesday, April 16, 2013
Cervid Industry Unites To Set Direction for CWD Reform and seem to ignore
their ignorance and denial in their role in spreading Chronic Wasting
Disease
Tuesday, June 05, 2012
Captive Deer Breeding Legislation Overwhelmingly Defeated During 2012
Legislative Session
Friday, August 31, 2012
COMMITTEE ON CAPTIVE WILDLIFE AND ALTERNATIVE LIVESTOCK and CWD 2009-2012 a
review
Monday, June 17, 2013
Early detection of chronic wasting disease prions in urine of
pre-symptomatic deer by real-time quaking-induced conversion assay
Wednesday, May 15, 2013
Intranasal Inoculation of White-Tailed Deer (Odocoileus virginianus) with
Lyophilized Chronic Wasting Disease Prion Particulate Complexed to
Montmorillonite Clay
Research Article
*** The potential impact of prion diseases on human health was greatly
magnified by the recognition that interspecies transfer of BSE to humans by beef
ingestion resulted in vCJD. While changes in animal feed constituents and
slaughter practices appear to have curtailed vCJD, there is concern that CWD of
free-ranging deer and elk in the U.S. might also cross the species barrier.
Thus, consuming venison could be a source of human prion disease. Whether BSE
and CWD represent interspecies scrapie transfer or are newly arisen prion
diseases is unknown. Therefore, the possibility of transmission of prion disease
through other food animals cannot be ruled out. There is evidence that vCJD can
be transmitted through blood transfusion. There is likely a pool of unknown size
of asymptomatic individuals infected with vCJD, and there may be asymptomatic
individuals infected with the CWD equivalent. These circumstances represent a
potential threat to blood, blood products, and plasma supplies.
UPDATED DATA ON 2ND CWD STRAIN
Wednesday, September 08, 2010 CWD PRION CONGRESS SEPTEMBER 8-11 2010
Tuesday, June 05, 2012
Captive Deer Breeding Legislation Overwhelmingly Defeated During 2012
Legislative Session
Friday, August 31, 2012
COMMITTEE ON CAPTIVE WILDLIFE AND ALTERNATIVE LIVESTOCK and CWD 2009-2012 a
review
Friday, August 24, 2012
Diagnostic accuracy of rectal mucosa biopsy testing for chronic wasting
disease within white-tailed deer (Odocoileus virginianus) herds in North America
The overall diagnostic specificity was 99.8%. Selective use of antemortem
rectal biopsy sample testing would provide valuable information during disease
investigations of CWD-suspect deer herds.
Tuesday, April 09, 2013
EFFICACY OF ANTEMORTEM RECTAL BIOPSIES TO DIAGNOSE AND ESTIMATE PREVALENCE
OF CHRONIC WASTING DISEASE IN FREE-RANGING COW ELK (CERVUS ELAPHUS NELSONI)
Monday, March 18, 2013
PROCEEDINGS ONE HUNDRED AND FIFTEENTH ANNUAL MEETING of the UNITED STATES
ANIMAL HEALTH ASSOCIATION September 29 – October 5, 2011
see updated 2012 RESOLUTIONS
The TSE prion disease survives ashing to 600 degrees celsius, that’s around
1112 degrees farenheit.
you cannot cook the TSE prion disease out of meat.
you can take the ash and mix it with saline and inject that ash into a
mouse, and the mouse will go down with TSE.
Prion Infected Meat-and-Bone Meal Is Still Infectious after Biodiesel
Production as well.
the TSE prion agent also survives Simulated Wastewater Treatment Processes.
IN fact, you should also know that the TSE Prion agent will survive in the
environment for years, if not decades.
you can bury it and it will not go away.
The TSE agent is capable of infected your water table i.e. Detection of
protease-resistant cervid prion protein in water from a CWD-endemic area.
it’s not your ordinary pathogen you can just cook it out and be done with.
that’s what’s so worrisome about Iatrogenic mode of transmission, a simple
autoclave will not kill this TSE prion agent.
New studies on the heat resistance of hamster-adapted scrapie agent:
Threshold survival after ashing at 600°C suggests an inorganic template of
replication
The infectious agents responsible for transmissible spongiform
encephalopathy (TSE) are notoriously resistant to most physical and chemical
methods used for inactivating pathogens, including heat. It has long been
recognized, for example, that boiling is ineffective and that higher
temperatures are most efficient when combined with steam under pressure (i.e.,
autoclaving). As a means of decontamination, dry heat is used only at the
extremely high temperatures achieved during incineration, usually in excess of
600°C. It has been assumed, without proof, that incineration totally inactivates
the agents of TSE, whether of human or animal origin.
Prion Infected Meat-and-Bone Meal Is Still Infectious after Biodiesel
Production
Histochemical analysis of hamster brains inoculated with the solid residue
showed typical spongiform degeneration and vacuolation. Re-inoculation of these
brains into a new cohort of hamsters led to onset of clinical scrapie symptoms
within 75 days, suggesting that the specific infectivity of the prion protein
was not changed during the biodiesel process. The biodiesel reaction cannot be
considered a viable prion decontamination method for MBM, although we observed
increased survival time of hamsters and reduced infectivity greater than 6 log
orders in the solid MBM residue. Furthermore, results from our study compare for
the first time prion detection by Western Blot versus an infectivity bioassay
for analysis of biodiesel reaction products. We could show that biochemical
analysis alone is insufficient for detection of prion infectivity after a
biodiesel process.
Detection of protease-resistant cervid prion protein in water from a
CWD-endemic area
The data presented here demonstrate that sPMCA can detect low levels of
PrPCWD in the environment, corroborate previous biological and experimental data
suggesting long term persistence of prions in the environment2,3 and imply that
PrPCWD accumulation over time may contribute to transmission of CWD in areas
where it has been endemic for decades. This work demonstrates the utility of
sPMCA to evaluate other environmental water sources for PrPCWD, including
smaller bodies of water such as vernal pools and wallows, where large numbers of
cervids congregate and into which prions from infected animals may be shed and
concentrated to infectious levels.
A Quantitative Assessment of the Amount of Prion Diverted to Category 1
Materials and Wastewater During Processing
Keywords:Abattoir;bovine spongiform encephalopathy;QRA;scrapie;TSE
In this article the development and parameterization of a quantitative
assessment is described that estimates the amount of TSE infectivity that is
present in a whole animal carcass (bovine spongiform encephalopathy [BSE] for
cattle and classical/atypical scrapie for sheep and lambs) and the amounts that
subsequently fall to the floor during processing at facilities that handle
specified risk material (SRM). BSE in cattle was found to contain the most oral
doses, with a mean of 9864 BO ID50s (310, 38840) in a whole carcass compared to
a mean of 1851 OO ID50s (600, 4070) and 614 OO ID50s (155, 1509) for a sheep
infected with classical and atypical scrapie, respectively. Lambs contained the
least infectivity with a mean of 251 OO ID50s (83, 548) for classical scrapie
and 1 OO ID50s (0.2, 2) for atypical scrapie. The highest amounts of infectivity
falling to the floor and entering the drains from slaughtering a whole carcass
at SRM facilities were found to be from cattle infected with BSE at rendering
and large incineration facilities with 7.4 BO ID50s (0.1, 29), intermediate
plants and small incinerators with a mean of 4.5 BO ID50s (0.1, 18), and
collection centers, 3.6 BO ID50s (0.1, 14). The lowest amounts entering drains
are from lambs infected with classical and atypical scrapie at intermediate
plants and atypical scrapie at collection centers with a mean of 3 × 10−7 OO
ID50s (2 × 10−8, 1 × 10−6) per carcass. The results of this model provide key
inputs for the model in the companion paper published here.
Saturday, December 15, 2012
*** Bovine spongiform encephalopathy: the effect of oral exposure dose on
attack rate and incubation period in cattle -- an update 5 December 2012
Thursday, June 6, 2013
BSE TSE PRION USDA FDA MAD COW FEED COMPLIANCE REPORT and NAI, OAI, and VAI
ratings as at June 5, 2013
Tuesday, June 4, 2013
*** INTERPRETING RESULTS OF FSIS VERIFICATION SAMPLING OF DOMESTIC BEEF
PRODUCT DERIVED FROM ADVANCED MEAT RECOVERY SYSTEMS (AMR01/FAMR01) FSIS Notice
38-12
Wednesday, February 16, 2011
IN CONFIDENCE
SCRAPIE TRANSMISSION TO CHIMPANZEES
IN CONFIDENCE
Sunday, April 18, 2010
SCRAPIE AND ATYPICAL SCRAPIE TRANSMISSION STUDIES A REVIEW 2010
Monday, April 25, 2011
Experimental Oral Transmission of Atypical Scrapie to Sheep
Volume 17, Number 5-May 2011
Sunday, December 12, 2010
EFSA reviews BSE/TSE infectivity in small ruminant tissues News Story 2
December 2010
Sunday, April 18, 2010
SCRAPIE AND ATYPICAL SCRAPIE TRANSMISSION STUDIES A REVIEW 2010
Thursday, December 23, 2010
Molecular Typing of Protease-Resistant Prion Protein in Transmissible
Spongiform Encephalopathies of Small Ruminants, France, 2002-2009
Volume 17, Number 1 January 2011
Thursday, November 18, 2010
Increased susceptibility of human-PrP transgenic mice to bovine spongiform
encephalopathy following passage in sheep
Monday, April 25, 2011
Experimental Oral Transmission of Atypical Scrapie to Sheep
Volume 17, Number 5-May 2011
Friday, February 11, 2011
Atypical/Nor98 Scrapie Infectivity in Sheep Peripheral Tissues
Thursday, March 29, 2012
atypical Nor-98 Scrapie has spread from coast to coast in the USA 2012
NIAA Annual Conference April 11-14, 2011San Antonio, Texas
Wednesday, April 4, 2012
20120402 - Breach of quarantine/Violation de la mise en quarantaine of an
ongoing Scrapie investigation
Michigan and California have had a high spike in Goat Scrapie cases,
compared to elsewhere ???
Tuesday, February 01, 2011
Sparse PrP-Sc accumulation in the placentas of goats with naturally
acquired scrapie
(Figure 6) including five goat cases in FY 2008 that originated from the
same herd in Michigan. This is highly unusual for goats, and I strenuously urge
that there should be an independent investigation into finding the common
denominator for these 5 goats in the same herd in Michigan with Scrapie. ...
Thursday, February 23, 2012
Atypical Scrapie NOR-98 confirmed Alberta Canada sheep January 2012
RESEARCH
Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 17, No. 5, May 2011
Experimental Oral Transmission of Atypical Scrapie to Sheep
Marion M. Simmons, S. Jo Moore,1 Timm Konold, Lisa Thurston, Linda A.
Terry, Leigh Thorne, Richard Lockey, Chris Vickery, Stephen A.C. Hawkins,
Melanie J. Chaplin, and John Spiropoulos
To investigate the possibility of oral transmission of atypical scrapie in
sheep and determine the distribution of infectivity in the animals’ peripheral
tissues, we challenged neonatal lambs orally with atypical scrapie; they were
then killed at 12 or 24 months. Screening test results were negative for
disease-specifi c prion protein in all but 2 recipients; they had positive
results for examination of brain, but negative for peripheral tissues.
Infectivity of brain, distal ileum, and spleen from all animals was assessed in
mouse bioassays; positive results were obtained from tissues that had negative
results on screening. These fi ndings demonstrate that atypical scrapie can be
transmitted orally and indicate that it has the potential for natural
transmission and iatrogenic spread through animal feed. Detection of infectivity
in tissues negative by current surveillance methods indicates that diagnostic
sensitivity is suboptimal for atypical scrapie, and potentially infectious
material may be able to pass into the human food chain.
SNIP...
Although we do not have epidemiologic evidence that supports the effi cient
spread of disease in the fi eld, these data imply that disease is potentially
transmissible under fi eld situations and that spread through animal feed may be
possible if the current feed restrictions were to be relaxed. Additionally,
almost no data are available on the potential for atypical scrapie to transmit
to other food animal species, certainly by the oral route. However, work with
transgenic mice has demonstrated the potential susceptibility of pigs, with the
disturbing fi nding that the biochemical properties of the resulting PrPSc have
changed on transmission (40). The implications of this observation for
subsequent transmission and host target range are currently unknown.
How reassuring is this absence of detectable PrPSc from a public health
perspective? The bioassays performed in this study are not titrations, so the
infectious load of the positive gut tissues cannot be quantifi ed, although
infectivity has been shown unequivocally. No experimental data are currently
available on the zoonotic potential of atypical scrapie, either through
experimental challenge of humanized mice or any meaningful epidemiologic
correlation with human forms of TSE. However, the detection of infectivity in
the distal ileum of animals as young as 12 months, in which all the tissues
tested were negative for PrPSc by the currently available screening and confi
rmatory diagnostic tests, indicates that the diagnostic sensitivity of current
surveillance methods is suboptimal for detecting atypical scrapie and that
potentially infectious material may be able to pass into the human food chain
undetected.
Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 17, No. 5, May 2011
why do we not want to do TSE transmission studies on chimpanzees $
5. A positive result from a chimpanzee challenged severly would likely
create alarm in some circles even if the result could not be interpreted for
man. I have a view that all these agents could be transmitted provided a large
enough dose by appropriate routes was given and the animals kept long enough.
Until the mechanisms of the species barrier are more clearly understood it might
be best to retain that hypothesis.
snip...
R. BRADLEY
1: J Infect Dis 1980 Aug;142(2):205-8
Oral transmission of kuru, Creutzfeldt-Jakob disease, and scrapie to
nonhuman primates.
Gibbs CJ Jr, Amyx HL, Bacote A, Masters CL, Gajdusek DC.
Kuru and Creutzfeldt-Jakob disease of humans and scrapie disease of sheep
and goats were transmitted to squirrel monkeys (Saimiri sciureus) that were
exposed to the infectious agents only by their nonforced consumption of known
infectious tissues. The asymptomatic incubation period in the one monkey exposed
to the virus of kuru was 36 months; that in the two monkeys exposed to the virus
of Creutzfeldt-Jakob disease was 23 and 27 months, respectively; and that in the
two monkeys exposed to the virus of scrapie was 25 and 32 months, respectively.
Careful physical examination of the buccal cavities of all of the monkeys failed
to reveal signs or oral lesions. One additional monkey similarly exposed to kuru
has remained asymptomatic during the 39 months that it has been under
observation.
snip...
The successful transmission of kuru, Creutzfeldt-Jakob disease, and scrapie
by natural feeding to squirrel monkeys that we have reported provides further
grounds for concern that scrapie-infected meat may occasionally give rise in
humans to Creutzfeldt-Jakob disease.
PMID: 6997404
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=6997404&dopt=Abstract
Recently the question has again been brought up as to whether scrapie is
transmissible to man. This has followed reports that the disease has been
transmitted to primates. One particularly lurid speculation (Gajdusek 1977)
conjectures that the agents of scrapie, kuru, Creutzfeldt-Jakob disease and
transmissible encephalopathy of mink are varieties of a single "virus". The U.S.
Department of Agriculture concluded that it could "no longer justify or permit
scrapie-blood line and scrapie-exposed sheep and goats to be processed for human
or animal food at slaughter or rendering plants" (ARC 84/77)" The problem is
emphasised by the finding that some strains of scrapie produce lesions identical
to the once which characterise the human dementias"
Whether true or not. the hypothesis that these agents might be
transmissible to man raises two considerations. First, the safety of laboratory
personnel requires prompt attention. Second, action such as the "scorched meat"
policy of USDA makes the solution of the acrapie problem urgent if the sheep
industry is not to suffer grievously.
snip...
76/10.12/4.6
Nature. 1972 Mar 10;236(5341):73-4.
Transmission of scrapie to the cynomolgus monkey (Macaca fascicularis).
Gibbs CJ Jr, Gajdusek DC.
Nature 236, 73 - 74 (10 March 1972); doi:10.1038/236073a0
Transmission of Scrapie to the Cynomolgus Monkey (Macaca fascicularis)
C. J. GIBBS jun. & D. C. GAJDUSEK
National Institute of Neurological Diseases and Stroke, National Institutes
of Health, Bethesda, Maryland
SCRAPIE has been transmitted to the cynomolgus, or crab-eating, monkey
(Macaca fascicularis) with an incubation period of more than 5 yr from the time
of intracerebral inoculation of scrapie-infected mouse brain. The animal
developed a chronic central nervous system degeneration, with ataxia, tremor and
myoclonus with associated severe scrapie-like pathology of intensive astroglial
hypertrophy and proliferation, neuronal vacuolation and status spongiosus of
grey matter. The strain of scrapie virus used was the eighth passage in Swiss
mice (NIH) of a Compton strain of scrapie obtained as ninth intracerebral
passage of the agent in goat brain, from Dr R. L. Chandler (ARC, Compton,
Berkshire).
*** I strenuously urge the USDA and the OIE et al to revoke the exemption
of the legal global trading of atypical Nor-98 scrapie TSE. ...TSS
Saturday, May 2, 2009
APHIS AND WHO PLAN TO EXEMPT THE ATYPICAL SCRAPIE NOR-98 FROM REGULATIONS
AT MEETING THIS MONTH
Monday, November 30, 2009
USDA AND OIE COLLABORATE TO EXCLUDE ATYPICAL SCRAPIE NOR-98 ANIMAL HEALTH
CODE
Thursday, December 20, 2012
OIE GROUP RECOMMENDS THAT SCRAPE PRION DISEASE BE DELISTED AND SAME OLD BSe
WITH BOVINE MAD COW DISEASE
5. A positive result from a chimpanzee challenged severely would likely
create alarm in some circles even if the result could not be interpreted for
man.
*** I have a view that all these agents could be transmitted provided a
large enough dose by appropriate routes was given and the animals kept long
enough.
Until the mechanisms of the species barrier are more clearly understood it
might be best to retain that hypothesis.
Wednesday, February 16, 2011
IN CONFIDENCE SCRAPIE TRANSMISSION TO CHIMPANZEES IN CONFIDENCE
reference...
RB3.20 TRANSMISSION TO CHIMPANZEES
1. Kuru and CJD have been successfully transmitted to chimpanzees but
scrapie and TME have not.
2. We cannot say that scrapie will not transmit to chimpanzees. There are
several scrapie strains and I am not aware that all have been tried (that would
have to be from mouse passaged material). Nor has a wide enough range of field
isolates subsequently strain typed in mice been inoculated by the appropriate
routes (i/c, ilp and i/v) :
3. I believe the proposed experiment to determine transmissibility, if
conducted, would only show the susceptibility or resistance of the chimpanzee to
infection/disease by the routes used and the result could not be interpreted for
the predictability of the susceptibility for man. Proposals for prolonged oral
exposure of chimpanzees to milk from cattle were suggested a long while ago and
rejected.
4. In view of Dr Gibbs' probable use of chimpazees Mr Wells' comments
(enclosed) are pertinent. I have yet to receive a direct communication from Dr
Schellekers but before any collaboration or provision of material we should
identify the Gibbs' proposals and objectives.
5. A positive result from a chimpanzee challenged severely would likely
create alarm in some circles even if the result could not be interpreted for
man. I have a view that all these agents could be transmitted provided a large
enough dose by appropriate routes was given and the animals kept long enough.
Until the mechanisms of the species barrier are more clearly understood it might
be best to retain that hypothesis.
6. A negative result would take a lifetime to determine but that would be a
shorter period than might be available for human exposure and it would still not
answer the question regarding mans' susceptibility. In the meantime no doubt the
negativity would be used defensively. It would however be counterproductive if
the experiment finally became positive. We may learn more about public reactions
following next Monday' s meeting.
R. Bradley 23 September 1990 CVO (+Mr Wells' comments) Dr T W A Little Dr B
J Shreeve 90/9.23/1.1.
see more here ;
Thursday, June 6, 2013
BSE TSE PRION USDA FDA MAD COW FEED COMPLIANCE REPORT and NAI, OAI, and VAI
ratings as at June 5, 2013
Greetings,
since our fine federal friends have decided not to give out any more
reports on the USA breaches of the feed ban and surveillance etc. for the BSE
TSE prion mad cow type disease in the USDA livestock, I thought I might attempt
it. I swear, I just don’t understand the logic of the SSS policy, and that
includes all of it. I assure you, it would be much easier, and probably better
for the FDA and the USDA INC., if they would simply put some kind of report out
for Pete’s sake, instead of me doing it after I get mad, because I am going to
put it all out there. the truth.
PLEASE BE ADVISED, any breach of any of the above classifications OAI, VAI,
RTS, CAN lead to breaches into the feed BSE TSE prion protocols, and CAN lead to
the eventual suspect tainted feed reaching livestock. please, if any USDA
official out there disputes this, please explain then how they could not.
paperwork errors can eventually lead to breaches of the BSE TSE prion mad cow
feed ban reaching livestock, or contamination and exposure there from, as well.
I would sure like to see the full reports of just these ;
4018 CHI-DO 3007091297 Rancho Cantera 2866 N Sunnyside Rd Kent IL
61044-9605 OPR FR, OF HP 11/26/2012 OAI Y
9367 3008575486 Rocky Ford Pet Foods 21693 Highway 50 East Rocky Ford CO
81067 OPR RE, TH HP 2/27/2013 OAI N
9446 DEN-DO 1713202 Weld County Bi Products, Inc. 1138 N 11th Ave Greeley
CO 80631-9501 OPR RE, TH HP 10/12/2012 OAI N
9447 DEN-DO 3002857110 Weld County Bi-Products dba Fort Morgan Pet Foods
13553 County Road 19 Fort Morgan CO 80701-7506 OPR RE HP 12/7/2011 OAI N
see full list of the fda mad cow bse feed follies, toward the bottom, after
a short brief update on the mad cow bse follies, and our good friend Lester
Crawford that was at the FDA.
ALSO, I would kindly like to comment on this FDA BSE/Ruminant Feed
Inspections Firms Inventory (excel format)4 format, for reporting these breaches
of BSE TSE prion protocols, from the extensive mad cow feed ban warning letters
the fda use to put out for each violations. simply put, this excel format sucks,
and the FDA et al intentionally made it this difficult to follow the usda fda
mad cow follies. this is an intentional format to make it as difficult as
possible to follow these breaches of the mad cow TSE prion safety feed
protocols. to have absolutely no chronological or numerical order, and to format
such violations in a way that they are almost impossible to find, says a lot
about just how far the FDA and our fine federal friends will go through to hide
these continued violations of the BSE TSE prion mad cow feed ban, and any
breaches of protocols there from. once again, the wolf guarding the henhouse $$$
NAI = NO ACTION INDICATED
OAI = OFFICIAL ACTION INDICATED
VAI = VOLUNTARY ACTION INDICATED
RTS = REFERRED TO STATE
Inspections conducted by State and FDA investigators are classified to
reflect the compliance status at the time of the inspection, based upon whether
objectionable conditions were documented. Based on the conditions found,
inspection results are recorded in one of three classifications:
OAI (Official Action Indicated) when inspectors find significant
objectionable conditions or practices and believe that regulatory sanctions are
warranted to address the establishment’s lack of compliance with the regulation.
An example of an OAI classification would be findings of manufacturing
procedures insufficient to ensure that ruminant feed is not contaminated with
prohibited material. Inspectors will promptly re-inspect facilities classified
OAI after regulatory sanctions have been applied to determine whether the
corrective actions are adequate to address the objectionable conditions.
VAI (Voluntary Action Indicated) when inspectors find objectionable
conditions or practices that do not meet the threshold of regulatory
significance, but warrant an advisory to inform the establishment that
inspectors found conditions or practices that should be voluntarily corrected.
VAI violations are typically technical violations of the 1997 BSE Feed Rule.
These violations include minor recordkeeping lapses or conditions involving
non-ruminant feeds.
NAI (No Action Indicated) when inspectors find no objectionable conditions
or practices or, if they find objectionable conditions, those conditions are of
a minor nature and do not justify further actions.
when sound science was bought off by junk science, in regards to the BSE
TSE prion mad cow type disease, by the USDA, CFIA, WHO, OIE, et al. $$$
when the infamous, and fraudulently USDA, FSIS, APHIS, FDA, gold card was
taken away that infamous day in December of 2003, all cards were off the table,
it was time to change the science, and change they did. ...tss
snip. ...please see full text ;
Thursday, June 6, 2013
BSE TSE PRION USDA FDA MAD COW FEED COMPLIANCE REPORT and NAI, OAI, and VAI
ratings as at June 5, 2013
Tuesday, June 11, 2013
Weld County Bi-Products dba Fort Morgan Pet Foods 6/1/12 significant
deviations from requirements in FDA regulations that are intended to reduce the
risk of bovine spongiform encephalopathy (BSE) within the United States
Thursday, February 14, 2013
*** The Many Faces of Mad Cow Disease Bovine Spongiform Encephalopathy BSE
and TSE prion disease
Thursday, June 13, 2013
Experimental interspecies transmission studies of the transmissible
spongiform encephalopathies to cattle: comparison to bovine spongiform
encephalopathy in cattle
10,000,000+ LBS. of PROHIBITED BANNED MAD COW FEED I.E. BLOOD LACED MBM IN
COMMERCE USA 2007
Date: March 21, 2007 at 2:27 pm PST
RECALLS AND FIELD CORRECTIONS: VETERINARY MEDICINES -- CLASS II
___________________________________
PRODUCT
Bulk cattle feed made with recalled Darling's 85% Blood Meal, Flash Dried,
Recall # V-024-2007
CODE
Cattle feed delivered between 01/12/2007 and 01/26/2007
RECALLING FIRM/MANUFACTURER
Pfeiffer, Arno, Inc, Greenbush, WI. by conversation on February 5, 2007.
Firm initiated recall is ongoing.
REASON
Blood meal used to make cattle feed was recalled because it was cross-
contaminated with prohibited bovine meat and bone meal that had been
manufactured on common equipment and labeling did not bear cautionary BSE
statement.
VOLUME OF PRODUCT IN COMMERCE
42,090 lbs.
DISTRIBUTION
WI
___________________________________
PRODUCT
Custom dairy premix products: MNM ALL PURPOSE Pellet, HILLSIDE/CDL Prot-
Buffer Meal, LEE, M.-CLOSE UP PX Pellet, HIGH DESERT/ GHC LACT Meal, TATARKA, M
CUST PROT Meal, SUNRIDGE/CDL PROTEIN Blend, LOURENZO, K PVM DAIRY Meal, DOUBLE B
DAIRY/GHC LAC Mineral, WEST PIONT/GHC CLOSEUP Mineral, WEST POINT/GHC LACT Meal,
JENKS, J/COMPASS PROTEIN Meal, COPPINI - 8# SPECIAL DAIRY Mix, GULICK, L-LACT
Meal (Bulk), TRIPLE J - PROTEIN/LACTATION, ROCK CREEK/GHC MILK Mineral,
BETTENCOURT/GHC S.SIDE MK-MN, BETTENCOURT #1/GHC MILK MINR, V&C DAIRY/GHC
LACT Meal, VEENSTRA, F/GHC LACT Meal, SMUTNY, A- BYPASS ML W/SMARTA, Recall #
V-025-2007
CODE
The firm does not utilize a code - only shipping documentation with
commodity and weights identified.
RECALLING FIRM/MANUFACTURER
Rangen, Inc, Buhl, ID, by letters on February 13 and 14, 2007. Firm
initiated recall is complete.
REASON
Products manufactured from bulk feed containing blood meal that was cross
contaminated with prohibited meat and bone meal and the labeling did not bear
cautionary BSE statement.
VOLUME OF PRODUCT IN COMMERCE
9,997,976 lbs.
DISTRIBUTION
ID and NV
END OF ENFORCEMENT REPORT FOR MARCH 21, 2007
Thursday, March 19, 2009
MILLIONS AND MILLIONS OF POUNDS OF MAD COW FEED IN COMMERCE USA WITH
ONGOING 12 YEARS OF DENIAL
Friday, September 4, 2009
FOIA REQUEST ON FEED RECALL PRODUCT 429,128 lbs. feed for ruminant animals
may have been contaminated with prohibited material Recall # V-258-2009
and all this was confirmed here ;
C O N F I R M E D
----- Original Message -----
From: "Terry S. Singeltary Sr."
To:
Sent: Thursday, November 05, 2009 9:25 PM
Subject: [BSE-L] re-FOIA REQUEST ON FEED RECALL PRODUCT contaminated with
prohibited material Recall # V-258-2009 and Recall # V-256-2009
Thursday, November 12, 2009
BSE FEED RECALL Misbranding of product by partial label removal to hide
original source of materials 2009
Tuesday, March 2, 2010
Animal Proteins Prohibited in Ruminant Feed/Adulterated/Misbranded Rangen
Inc 2/11/10 USA
Monday, March 1, 2010
ANIMAL PROTEIN I.E. MAD COW FEED IN COMMERCE A REVIEW 2010
Monday, April 5, 2010
Update on Feed Enforcement Activities to Limit the Spread of BSE April 5,
2010
Saturday, July 23, 2011
CATTLE HEADS WITH TONSILS, BEEF TONGUES, SPINAL CORD, SPECIFIED RISK
MATERIALS (SRM's) AND PRIONS, AKA MAD COW DISEASE
Saturday, November 6, 2010
TAFS1 Position Paper on Position Paper on Relaxation of the Feed Ban in the
EU Berne, 2010 TAFS
INTERNATIONAL FORUM FOR TRANSMISSIBLE ANIMAL DISEASES AND FOOD SAFETY a
non-profit Swiss Foundation
Archive Number 20101206.4364 Published Date 06-DEC-2010 Subject
PRO/AH/EDR> Prion disease update 2010 (11)
PRION DISEASE UPDATE 2010 (11)
the new BSE TSE PRION MAD COW risk category the OIE gave the USA, puts
everyone around the globe at more risk of a tse prion mad cow type disease now.
in my opinion, this new risk category was bought and paid for by your local
cattle dealer, via fraud.
IT is of my opinion, that the OIE and the USDA et al, are the soul reason,
and responsible parties, for Transmissible Spongiform Encephalopathy TSE prion
diseases, including typical and atypical BSE, typical and atypical Scrapie, and
all strains of CWD, and human TSE there from, spreading around the globe.
I have lost all confidence of this organization as a regulatory authority
on animal disease, and consider it nothing more than a National Trading
Brokerage for all strains of animal TSE, just to satisfy there commodity. AS i
said before, OIE should hang up there jock strap now, since it appears they will
buckle every time a country makes some political hay about trade protocol,
commodities and futures. IF they are not going to be science based, they should
do everyone a favor and dissolve there organization.
JUST because of low documented human body count with nvCJD and the long
incubation periods, the lack of sound science being replaced by political and
corporate science in relations with the fact that science has now linked some
sporadic CJD with atypical BSE and atypical scrapie, and the very real threat of
CWD being zoonosis, I believed the O.I.E. has failed terribly and again, I call
for this organization to be dissolved. ...
IN A NUT SHELL ;
(Adopted by the International Committee of the OIE on 23 May 2006)
11. Information published by the OIE is derived from appropriate
declarations made by the official Veterinary Services of Member Countries. The
OIE is not responsible for inaccurate publication of country disease status
based on inaccurate information or changes in epidemiological status or other
significant events that were not promptly reported to the Central Bureau,
USDA INC
Thursday, May 30, 2013
World Organization for Animal Health (OIE) has upgraded the United States'
risk classification for mad cow disease to "negligible" from "controlled", and
risk further exposing the globe to the TSE prion mad cow type disease
U.S. gets top mad-cow rating from international group and risk further
exposing the globe to the TSE prion mad cow type disease
sporadic CJD has now been linked to atypical BSE, atypical Scrapie, and
don’t count CWD, typical scrapie, or typical BSE (Collinge et al) out just yet.
...
Monday, October 10, 2011
EFSA Journal 2011 The European Response to BSE: A Success Story
snip...
EFSA and the European Centre for Disease Prevention and Control (ECDC)
recently delivered a scientific opinion on any possible epidemiological or
molecular association between TSEs in animals and humans (EFSA Panel on
Biological Hazards (BIOHAZ) and ECDC, 2011).
*** This opinion confirmed Classical BSE prions as the only TSE agents
demonstrated to be zoonotic so far but the possibility that a small proportion
of human cases so far classified as "sporadic" CJD are of zoonotic origin could
not be excluded.
Moreover, transmission experiments to non-human primates suggest that some
TSE agents in addition to Classical BSE prions in cattle (namely L-type Atypical
BSE, Classical BSE in sheep, transmissible mink encephalopathy (TME) and chronic
wasting disease (CWD) agents) might have zoonotic potential.
snip...
Thursday, August 12, 2010
Seven main threats for the future linked to prions
First threat
The TSE road map defining the evolution of European policy for protection
against prion diseases is based on a certain numbers of hypotheses some of which
may turn out to be erroneous. In particular, a form of BSE (called atypical
Bovine Spongiform Encephalopathy), recently identified by systematic testing in
aged cattle without clinical signs, may be the origin of classical BSE and thus
potentially constitute a reservoir, which may be impossible to eradicate if a
sporadic origin is confirmed.
***Also, a link is suspected between atypical BSE and some apparently
sporadic cases of Creutzfeldt-Jakob disease in humans. These atypical BSE cases
constitute an unforeseen first threat that could sharply modify the European
approach to prion diseases.
Second threat
snip...
Rural and Regional Affairs and Transport References Committee
The possible impacts and consequences for public health, trade and
agriculture of the Government's decision to relax import restrictions on beef
Final report June 2010
2.65 At its hearing on 14 May 2010, the committee heard evidence from Dr
Alan Fahey who has recently submitted a thesis on the clinical neuropsychiatric,
epidemiological and diagnostic features of Creutzfeldt-Jakob disease.48 Dr Fahey
told the committee of his concerns regarding the lengthy incubation period for
transmissible spongiform encephalopathies, the inadequacy of current tests and
the limited nature of our current understanding of this group of
diseases.49
2.66 Dr Fahey also told the committee that in the last two years a link has
been established between forms of atypical CJD and atypical BSE. *** Dr Fahey
said that: They now believe that those atypical BSEs overseas are in fact
causing sporadic Creutzfeldt-Jakob disease. They were not sure if it was due to
mad sheep disease or a different form. If you look in the textbooks it looks
like this is just arising by itself. But in my research I have a summary of a
document which states that there has never been any proof that sporadic
Creutzfeldt-Jakob disease has arisen de novo-has arisen of itself. There is no
proof of that. The recent research is that in fact it is due to atypical forms
of mad cow disease which have been found across Europe, have been found in
America and have been found in Asia. These atypical forms of mad cow disease
typically have even longer incubation periods than the classical mad cow
disease.50
Wednesday, March 31, 2010
Atypical BSE in Cattle
To date the OIE/WAHO assumes that the human and animal health standards set
out in the BSE chapter for classical BSE (C-Type) applies to all forms of BSE
which include the H-type and L-type atypical forms. This assumption is
scientifically not completely justified and accumulating evidence suggests that
this may in fact not be the case. Molecular characterization and the spatial
distribution pattern of histopathologic lesions and immunohistochemistry (IHC)
signals are used to identify and characterize atypical BSE. Both the L-type and
H-type atypical cases display significant differences in the conformation and
spatial accumulation of the disease associated prion protein (PrPSc) in brains
of afflicted cattle. Transmission studies in bovine transgenic and wild type
mouse models support that the atypical BSE types might be unique strains because
they have different incubation times and lesion profiles when compared to C-type
BSE.
When L-type BSE was inoculated into ovine transgenic mice and Syrian
hamster the resulting molecular fingerprint had changed, either in the first or
a subsequent passage, from L-type into C-type BSE. In addition, non-human
primates are specifically susceptible for atypical BSE as demonstrated by an
approximately 50% shortened incubation time for L-type BSE as compared to
C-type. Considering the current scientific information available, it cannot be
assumed that these different BSE types pose the same human health risks as
C-type BSE or that these risks are mitigated by the same protective
measures.
This study will contribute to a correct definition of specified risk
material (SRM) in atypical BSE. The incumbent of this position will develop new
and transfer existing, ultra-sensitive methods for the detection of atypical BSE
in tissue of experimentally infected cattle.
add all the above up, and then ponder iatrogenic CJD there from $$$
Greetings Dr. Paul Van Buynder et al @ FraserHealth,
Sadly, in the year 2013, we are still going by science that is 3 decades
old, to manage risk factors from the many different strains of the TSE prion
disease.
Dr. Paul Van Buynder et al state ;
> I want to be clear there is absolutely no evidence that these three
confirmed or probable cases are linked to food consumption.
when in reality, you have no clue Sir.
sporadic CJD is not a single strain of the TSE prion sporadic phenotypes
(there are many, and they are mounting).
sporadic CJD has now been linked to atypical BSE, and atypical Scrapie, and
many reputable scientist around the globe are especially concerned with the CWD
of cervids, and it’s different strains, which we now know there are more
strains.
the UKBSEnvCJD only theory is a false myth, and proven to be so.
also, all iatrogenic CJD is, is sporadic CJD until route and source of the
TSE agent is confirmed.
what fuels this madness, and the spread of this disease, are Doctors and
officials that continue to spread this junk science.
you are part of the problem, in my opinion, and I mean no disrespect Sir.
if I still sound angry, I am, 15+ years later.
I have wasted 15 years daily following the science as it emerges with the
TSE prion disease, and documenting it. I hope you take the time to read some of
it.
no need to reply, most never do, but I urge you to educate yourself on this
topic of the TSE prion disease, and cease spreading the false science that
continues to fuel the spread of this TSE prion disease. ...
thank you, kind regards,
terry
Saturday, June 15, 2013
Canada Fraser Health Statement on Creutzfeldt-Jakob Disease outbreak
TSS
